孕早期产前筛查孕妇血清ADAM12-S中位数倍数校正方法的建立  

Establishment of adjusted value for multiple of median of ADAM12-S concentration in prenatal serum screening during early pregnancy

作　　者：蒋涛[1] 吕伶[1] 张晓娟[1] 孙云[1] 徐倩君[1] 许争峰[1] 

机构地区：[1]南京医科大学附属南京妇幼保健院产前诊断中心,南京210004

出　　处：《临床检验杂志》2011年第1期50-52,共3页Chinese Journal of Clinical Laboratory Science

基　　金：南京市医学科技发展项目(ZKX08001;ZKX06011);南京市科技局项目(200801094)

摘　　要：目的建立孕早期产前筛查孕妇血清解整合素金属蛋白酶12分泌型(a disintegrin and metalloprotease,ADAM12-S)中位数倍数的校正方法。方法随机选择2008年在本中心自愿参加孕早期唐氏综合征(DS)产前筛查并经随访证实分娩健康儿的孕妇孕8～13周血清样本577份,用时间分辨荧光免疫分析法检测血清ADAM12-S浓度。用SPSS 15.0软件构建ADAM12-S与孕龄间的回归关系,选最优模型计算中位浓度,得到ADAM12-S中位数倍数(multiple of the median,MoM)值。探讨MoM值与体重的关系,选取最优模型求出经体重调整后的MoM值。将ADAM12-S初始浓度与消除孕周和体重影响校正后的最终MoM值进行曲线拟合,选取最优模型最终得到ADAM12-S初始浓度与校正后MoM值的关系方程。结果 ADAM12-S中位数(Y)与孕周(t)呈正相关,经s曲线模型拟合后的中位数方程为:Y=e8.412-24.534/t;ADAM12-S MoM值中位数(Y)与体重中位数(z)存在负相关,经指数模型拟合后的方程为:Y=1.603*e-0.009*z;ADAM12-S初始浓度(X)与孕周和体重校正后的最终MoM值(Y)的关系方程为Y=0.036*X0.552。结论 ADAM12-S初始浓度与最终MoM值的关系方程的建立,对临床开展ADAM12-S筛查DS妊娠的研究有重要意义。Objective To establish the adjusted value for multiple of the median（MOM） of a disintegrin and metalloprotease 12-secreting-type（ADAM12-S） in prenatal serum screening for Down′s syndrome.Methods The parturients,who were voluntary participants of prenatal screening program for Down′s syndrome during 2008 and were confirmed to labor healthy newborn by follow-up visit,were randomly enrolled in this study.Totally 577 serum samples of the first trimester（from 8 to 13 weeks） were collected and the concentration of ADAM12-S was determined by time-resolved fluorimmunoassay（TRFIA）.The regressive relationship between concentration of ADAM12-S and gestational age was analyzed with SPSS 15.0 software and then the optimal model was selected for the calculation of MOM value of ADAM12-S.The MOM value was adjusted on the basis of the body weight of parturients.The curve fitting of ADAM12-S concentration and the adjusted MOM value were conducted and the model was optimized for the equation by eliminating the influence of gestational age and body weight.Results A positive correlation between the MOM value of ADAM12-S（Y） and gestational age（t） was shown,and the equation was Y=e8.412-24.534/t,while a negative correlation between MOM value of ADAM12-S（Y） and body weight of parturients（z） was found,and the equation was Y=1.603＊e-0.009＊z.The equation of concentration of ADAM12-S（Y） and the adjusted MOM value（X） was Y=0.036＊X0.552.Conclusions The equation between the initial concentration of ADAM12-S and the adjusted MOM value was established.It should be considerably important for the screening program of Down′s syndrome in primary clinical hospital for newborn.

关 键 词：解整合素金属蛋白酶12分泌型 唐氏综合征 产前筛查 

分 类 号：R596.1[医药卫生—内科学] R446.1[医药卫生—临床医学]