荧光双向凝胶电泳分析温热处理对K562细胞系蛋白质表达谱的影响  

Effects of lukewarm treatment on the expressing spectrum of protein in K562 cells analyzed by fluorescent 2-DE

作　　者：赵亮[1] 刘亚伟[2] 孙学刚 

机构地区：[1]南方医科大学南方医院病理科基础医学院病理学系,广州市510515 [2]南方医科大学南方医院病理科基础医学院病理生理学教研室,广州市510515 [3]中医药学院分子生物学实验室

出　　处：《实用医学杂志》2011年第2期174-176,共3页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目(编号:30901792);南方医科大学基础医学院院长基金资助项目(编号:JC0802)

摘　　要：目的:观察温热处理对K562细胞蛋白质表达谱的影响。方法:将培养获得的K562细胞在41℃恒温处理1h,以37℃作为对照,采用荧光双向凝胶电泳技术分离全细胞蛋白质,分子成像仪获取图像后软件分析差异表达的蛋白质点。结果:温热处理K562细胞与对照组细胞蛋白质表达谱分析比较差异表达蛋白质点数为65个,其中温热处理后表达上调的蛋白质点27个,下调的蛋白质点38个。结论:研究将为阐明热疗的分子基础提供可靠的实验依据,随着研究的深入必将推动基于热疗的肿瘤生物治疗的更大发展和广泛的临床应用。Objective To investigate the effects of lukewarm treatment on the expressing spectrum of protein in K562 cells. Methods As compared with those treated under 37℃,cultured K562 cells were treated under 41℃ ior 1 hour,then whole cellular proteins were separated by fluorescent 2-DE.Images obtained by molecular imaging were analyzed by software to discover different expression protein spots. Results 65 protein spots were differentiall？ expressed between K562 eells in the two groups, among which, 27 spots were up-regulated and 38 spots were down-regulated in K562 cells after lukewarm treatment. Conclusion This study paside ide the experimental foundation fur elucidating molecular mechanism of thermotherapy.

关 键 词：蛋白质组学 热疗 K562细胞 温热处理 

分 类 号：R730.5[医药卫生—肿瘤]