细胞连续亲和-HPLC测定8种蟾蜍甾烯与MGC-803细胞亲和量及抗肿瘤活性相关分析  

Cell continuous extraction-HPLC determination biological affinity of 8 bufadienolides on MGC-803 cells and their correlation with anti-tumor activities

作　　者：蒋洁君[1] 尤奋强[2] 马宏跃[1] 周婧[1] 张军峰[1] 詹臻[1] 唐于平[1] 段金廒[1] 丁安伟[1] 

机构地区：[1]南京中医药大学江苏省方剂研究重点实验室,江苏南京210046 [2]南京中医药大学昆山附属医院,江苏昆山251300

出　　处：《中国中药杂志》2011年第2期205-208,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学青年基金项目(30901894);教育部新教师基金项目(20093237120013);霍英东教育基金会第十二届高等院校青年教师基金项目(121044);江苏省中医药局项目(LZ09017);南京中医药大学青年自然基金项目(09XZR21)

摘　　要：目的:采用细胞连续亲和研究蟾蜍甾烯(Bu)与肿瘤细胞的生物亲和作用,分析其与抗肿瘤活性的相关性。方法:将蟾酥氯仿提取物与胃癌细胞MGC-803混合培养,高效液相色谱测定上清液和细胞内8种Bu的含量,计算亲和率。结果:亲和后细胞上清液中Bu总减少率、细胞内游离Bu的含量百分率和两者差值(与细胞裂解物的亲和率)、抑制MGC-803活性(1/IC50)相关系数r分别是0.82(P<0.05),-0.04,0.83(P<0.05)。结论:8种蟾蜍甾烯与MGC-803发生不同程度亲和。其与肿瘤细胞或细胞裂解物的亲和率与抗肿瘤活性有显著相关性。Objective： To study the bioaffinity between 8 bufadienolides（Bu） and tumor cells and analyze the correlation between the bioaffinity and the anti-tumor activities of Bu. Method： Mix and cultivate the chloroform extract of Chansu and MGC-803. Measure the content of 8 Bu in supematant and cells using HPLC and calculate their affinity rate. Result： The coefficient correlation between the decrease of Bu in cell supernatant after affinity and its MGC-803 restrictive activities, and between the cotent percentage of the free Bu in free cells with its MGC-803 restrictive activities, and between the difference between the decrease and the percentage and its MGC-803 restrictive activities is r =0. 82 （P 〈0. 05） ,r =-0. 04 and r =0. 83（P 〈0. 05） respectively. Conclusion： Eight Bu have different levels of affinity with MGC-803 which correlate with their anti-tumor activities.

关 键 词：蟾蜍甾烯 MGC-803 细胞连续亲和 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]