尿素柱层析分离共轭亚油酸甲酯异构体的研究  

Separation of conjugated methyl linoleate isomer by urea column chromatography

作　　者：郝建秀[1,2] 贾时宇[1] 邓天昇 侯相林[1] 

机构地区：[1]中国科学院山西煤炭化学研究所,太原030001 [2]中国科学院研究生院,北京100039

出　　处：《中国油脂》2011年第1期9-12,共4页China Oils and Fats

摘　　要：利用负载型尿素层析柱对共轭亚油酸甲酯异构体进行分离。考察了固定相中甲醇残留量、尿素硅胶质量比、固定相结晶温度、层析温度及洗脱剂种类对分离效果的影响。结果表明：当固定相中甲醇残留量为3％～5％，尿素硅胶质量比为1：1，固定相结晶温度0～8℃，层析温度0℃，洗脱剂为石油醚时，经过层析分离反10，顺12共轭亚油酸甲酯的含量从样品中的33．94％提高到57．69％。The conjugated methyl linoleate isomer was separated by urea column chromatography , and the factors influencing the separation effects of conjugated methyl linoleate isomer were investigated. The resuits showed that the content of t10,c12 conjugated methyl linoleate could be improved from 33.94% to 57.69% at the followed conditions： methanol residue in fixed phase 3% -5% , mass ratio of urea to silica gel 1 ： 1, crystallization temperature 0 - 8℃operation temperature 0 ℃, eluent petroleum.

关 键 词：尿素柱层析 共轭亚油酸甲酯 异构体 

分 类 号：TQ645[化学工程—精细化工] TQ028