孔圣枕中丹对SHR大鼠前额叶皮质及纹状体TH、DAT蛋白和基因表达的影响  

Effect of Kongsheng Zhenzhong Pill on TH and DAT Protein and Gene Expression in Prefrontal Cortex and Corpus Striatum of SHR Rats

作　　者：徐谦[1] 陈晓刚[2] 谭丽丽[2] 许双虹[2] 罗荣敬[1] 关莉[1] 蒋红兰[2] 李宜瑞[2] 

机构地区：[1]广州中医药大学,广州510405 [2]广州中医药大学第一附属医院,广州510405

出　　处：《中药新药与临床药理》2011年第1期25-29,共5页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：国家自然科学基金资助项目(30772821)

摘　　要：目的探讨孔圣枕中丹对注意缺陷多动障碍(ADHD)动物模型SHR大鼠前额叶皮质、纹状体TH、DAT蛋白和基因表达的影响。方法 90只SHR雄性大鼠分为3批,每批大鼠随机分为3组,分别用孔圣枕中丹、盐酸哌甲酯和生理盐水灌胃28 d,采用免疫组化法、WESTERN BLOT、原位杂交法和荧光定量PCR检测其前额叶皮质、纹状体TH、DAT蛋白和基因的表达。结果 SHR大鼠前额叶皮质及纹状体TH蛋白和基因的表达,孔圣枕中丹组高于盐酸哌甲酯组,盐酸哌甲酯组又高于生理盐水对照组;DAT蛋白和基因的表达孔圣枕中丹组低于盐酸哌甲酯组,盐酸哌甲酯组又低于生理盐水对照组,与对照组比较,P<0.05,有统计学意义。结论孔圣枕中丹可能通过影响调控中枢前额叶皮质-基底神经节环路的DA神经信号传导而治疗ADHD。Objective To investigate the effect of Kongsheng Zhenzhong Pill on TH and DAT protein and gene expression in prefrontal cortex and corpus striatum of rats with attention deficit hyperactivity disorder（ADHD）. Methods Thirty SHR rats were randomly divided into three groups. The three groups were given gastric garage with Kongsheng Zhenzhong Pill, methylphenidate hydrochloride, normal saline, respectively. The treatment lasted 28 days. Immunohistochemical staining, Western blotting, in situ hybridization and fluorescence quantitative PCR were used to detect TH and DAT protein and gene expression in prefrontal cortex and corpus striatum of rats. Results TH protein and gene expression in the rat prefrontal cortex and corpus striatum of Kongsheng Zhenzhong Pill group was higher than that in methylphenidate hydrochloride group, and TH expression in methylphenidate hydrochloride group was higher than that in the saline control group. DAT protein and gene expression in Kongsheng Zhenzhong Pill group was lower than that in methylphenidate hydrochloride group, while methylphenidate hydrochloride group had lower DAT protein and gene expression than that in the saline control group （P 〈 0.05）. Conclusion The therapeutic mechanism of Kongsheng Zhenzhong Pill for ADHD is probably related with affecting the central regulation of the prefrontal cortex-basal ganglia loop of the DA nerve signal transduction.

关 键 词：孔圣枕中丹 SHR大鼠 前额叶皮质 纹状体 TH DAT 蛋白 基因 

分 类 号：R285.5[医药卫生—中药学]