山柰酚对照品的标定  

Standardization of Kaempferol Reference Substance

作　　者：谢春燕[1] 徐新军[1] 刘群娣[1] 闫李丽[1] 谢晓玲 杨得坡[1] 

机构地区：[1]中山大学药学院,广州510006 [2]广州中一药业有限公司,广州510000

出　　处：《中药新药与临床药理》2011年第1期99-102,共4页Traditional Chinese Drug Research and Clinical Pharmacology

基　　金：广东省科技厅国际合作基金(2008A050200005);科技部国际合作项目(2009DFA31230)

摘　　要：目的标定山柰酚对照品。方法采用高效液相色谱二极管阵列检测(DAD)测定山柰酚样品纯度。结果经高效液相色谱紫外检测,以面积归一化法和自身稀释对照法计算山柰酚样品纯度,均达98.0%以上。结论多种分析方法互相印证,表明该批样品达到中药化学对照品纯度要求,所用分析方法可靠。Objective To standardize the kaempferol reference substance. Methods HPLC- diode array detection （DAD）was applied to determine the purity of kaempferol, and then the purity of kaempferol were calculated both by area normalization method and self-contrasted dilution method. Results The results of normalization method and self-contrasted dilution method showed that the purity was over 98.0 %. Conclusion Purity of this batch of kaempferol could reach the requirement of chemical reference standards for traditional Chinese medicine by confirmation of various analytical methods, and the methods used for the assay of kaempferol are practical.

关 键 词：山柰酚 高效液相色谱法 面积归一化法 自身对照法 薄层色谱法 

分 类 号：R284.1[医药卫生—中药学]