大鼠腹腔同种异体异位心脏移植模型的建立与改进  

Establishment and improvement of allogeneic rat models of heterotopic cardiac transplantation

作　　者：郭宇[1] 叶小鸣[1] 张彤[2,3] 伍庄 华学锋[5] 张琪[2,3] 陈规划[2,3] 

机构地区：[1]中山大学附属第三医院特诊医疗中心,广州510630 [2]中山大学附属第三医院肝移植中心 [3]中山大学器官移植研究所广东省器官移植研究中心 [4]武汉钢铁公司总医院 [5]中山大学附属第三医院肾移植科

出　　处：《器官移植》2011年第1期18-22,45,共6页Organ Transplantation

基　　金：国家重点基础研究发展计划(973计划)课题(2009CB522404);国家十一五科技重大专项课题(2008ZX10002-026);广东省科技计划项目重大专项(2007A032000001)

摘　　要：目的建立并改进大鼠腹腔同种异体异位心脏移植模型,探讨其可行性及安全性。方法对100只Wistar大鼠腹腔同种异体异位心脏移植模型的麻醉方法、供心灌注切取、受体血管准备、吻合方法等进行了改良,术后统计手术时间及手术成功率。结果供体准备、供心摘取时间共(13±3)min,受体血管准备时间(8±2)min,受体腹主动脉吻合时间(10±2)min,受体下腔静脉吻合时间(7±3)min,供心冷缺血时间(30±6)min。手术成功大鼠恢复血供到开始心室纤颤时间(5±2)s,心室纤颤期为(10±3)s。100只中,成功94只,成功率为94%,死亡6只,包括术中麻醉意外死亡3只,术中误伤心耳出血死亡2只,肺血管结扎处出血死亡1只。结论大鼠腹腔同种异体异位心脏移植模型的操作方法经过改进后,降低了手术难度,手术成功率较高。Objective To establish allogeneic heterotopic cardiac transplantation models in rats with modified surgical technique and to evaluate the feasibility and safety of the applied technique.Methods 100 rats of heterotopic cardiac transplantation were performed with modified technique of anesthesia,heart-harvesting,receptor vascular preparation and vascular anastomosis.The operation time and success rate were recorded and calculated.Results The process of donor-preparation and heart-harvesting took about（13±3）minutes and receptor vascular preparation took around（8±2）min.Abdominal aorta anastomosis time was（10±2）minutes and inferior vena cava anastomosis time was（7±3）minutes.The average cold ischemia time was（30±6）minutes.The recipient rats usually took（5±2）seconds to restart the blood supply of heart and to start ventricular fibrillation after operation.The average ventricular fibrillation time was（10±3）seconds.In 100 cases,94 recipient rats（94 %）underwent successful heterotopic cardiac transplantation.Three fatal cases died of anesthesia accident,two fatal cases died of atria hurt and one fatal case died of hemorrhage of lung vessel ligation.Conclusion This improved method can increase the success rate of transplantation and make the operation easier.

关 键 词：心脏移植 同种移植 异位移植 大鼠 手术 动物模型 

分 类 号：R-332[医药卫生]