分散液液微萃取-高效液相色谱法测定食品中的维生素A和E  

Dispersive liquid-liquid microextraction coupled with high performance liquid chromatography for the determination of vitamins A and E in food

作　　者：姜兰芳[1] 周光明[1] 李艳艳[1] 

机构地区：[1]发光与实时分析教育部重点实验室,西南大学化学化工学院,重庆400715

出　　处：《分析试验室》2011年第2期61-65,共5页Chinese Journal of Analysis Laboratory

基　　金：国家科技重大专项(2008ZX07315);发光与实时分析重庆市重点实验室(西南大学)(CSTC,2006CA8006);重庆市自然科学基金项目(CSTC2,007BB5370)资助

摘　　要：以维生素A和E萃取率的平均值为检测指标,采用L18(37)正交试验法考察了萃取剂的类型、用量、分散剂的类型、用量以及萃取时间和离子强度6个影响因素,利用高效液相色谱法测定维生素含量。维生素A和E在0.23～174μg/mL范围内线性关系良好;定性检出限分别为3.26 ng和7.6 ng(S/N=3);相对标准偏差为0.68%～3.59%(n=6);浓缩因子为75～90。该方法应用于食品分析,加标回收率为90.6%～96.7%,RSD为1.8%～3.2%(n=6)。The dispersive liquid-liquid microextraction coupled with high performance liquid chromatography （ DLLM E-HPLC） , has been developed for the extraction and determination of vitamins A and E in food. The six relevant factors, such as type and volume of dispersion agent and extraction solvents, the extraction time and ionic strength were investigated and optimized by the Lls （37） orthogonal design with the average extraction recoveries of vitamins A and E as the index determined by HPLC. Under the optimized extraction conditions, the reliability of the proposed method was evaluated. A good linear relationship was obtained in the range of 0.23 174 μg/mL for both vitamin A and vitamin E. The limits of detection were 3.26 ng, 7.6 ng （S/N = 3 ）, respectively. The relative standard deviations were 0. 68% - 3.59% （ n = 6 ）. The enrichment factors ranged from 75 to 90. The developed method was applied to the determination of vitamins A and E in food, the average recoveries were 90. 6% -96. 7% with RSD of 1.78% -3.2% （n =6）.

关 键 词：分散液液微萃取 高效液相色谱法 维生素 食品 

分 类 号：O657.7[理学—分析化学]