三七中人参皂苷Rh_1的分离与纯化  

Separation and purification of ginsenoside Rh_1 in Panax notoginseng

作　　者：李龙华[1,2] 富瑶瑶[1] 石岭[1] 许郁峰[1] 鱼红闪[1] 金凤燮[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034 [2]大连生生绿谷生物工程有限公司,辽宁大连116023

出　　处：《大连工业大学学报》2011年第1期23-25,共3页Journal of Dalian Polytechnic University

基　　金：国家自然科学基金资助项目(30470055);辽宁省教育厅创新团队项目(2007T006)

摘　　要：为了得到纯净的、具有较高生理和药理活性的单体人参皂苷Rh1,从三七中提取总皂苷,并对三七总皂苷进行分离纯化获得人参皂苷Rh1单体。分别经过乙醇浸提、石油醚脱脂、水饱和正丁醇萃取、大孔吸附树脂脱糖脱色及硅胶层析,从三七总皂苷中分离得到人参皂苷Rh1。结果表明,1 000 g三七干燥根经醇提、脱脂、萃取和脱糖脱色可制得三七总皂苷76.2 g,提取率为7.62%。25 g三七总皂苷以氯仿-甲醇溶液[V(氯仿)∶V(甲醇)=8.5∶1.5]作为流动相洗脱,经硅胶层析得人参皂苷Rh1单体0.36 g,得率为1.44%,高效液相色谱检测其纯度为66.08%。The ginsenoside Rh1 in Panax notoginseng was isolated by silica gel chromatography.76.2 g total notoginsenoside was obtained from 1 kg Panax notoginseng,the yield of total notoginsenoside was about 7.62%.0.36 g ginsenoside Rh1 was separated from 25 g total notoginsenoside by silica gel chromatography using chloroform and methanol at 8.5∶1.5 as the mobile phase,the yield of ginsenoside Rh1 was 1.44%,the purity was 66.08% detecting by HPLC.

关 键 词：三七 人参皂苷 硅胶层析 高效液相色谱 

分 类 号：TS201.25[轻工技术与工程—食品科学] Q946.83[轻工技术与工程—食品科学与工程]