核盘菌和草酸诱导下的油菜几种酶活力的变化分析  

Analysis of several enzymes activity induced by Sclerotinia sclerotiorum and oxalic acid in rapeseed

作　　者：毛玮[1] 侯英敏[1] 刘志文[1] 

机构地区：[1]大连工业大学生物工程学院,辽宁大连116034

出　　处：《大连工业大学学报》2011年第1期39-42,共4页Journal of Dalian Polytechnic University

基　　金：辽宁省教育厅科学技术研究项目(2009S011);大连市科学技术基金项目(2010J21DW015)

摘　　要：油菜菌核病是油菜生产中的重要病害之一,为了进一步探讨菌核病的致病机理和抗病机制,采用草酸浸叶和接种核盘菌菌丝两种方法分别处理甘蓝型油菜叶片,确定草酸最佳浓度为3 mmol/L,分析叶片中过多酚氧化酶(PPO)、氧化物酶(POD)、纤维素酶和几丁质酶的活力变化,发现草酸和核盘菌都可以诱导防御酶PPO和POD活力升高,可增强植物的抗病性;纤维素酶在核盘菌诱导96 h后活力明显上升,说明纤维素酶与核盘菌致病性密切相关;核盘菌诱导72 h后几丁质酶活力上升明显,说明几丁质酶与防卫反应有关。Sclerotinia sclerotiorum was one of the important diseases during the cultivation of Brassica napus.Two methods of dipping leaf into oxalic acid solution and inoculation with S.sclerotiorum were used in this study for discussing the pathogenesis and disease resistance mechanism.The optimal concentration of oxalic acid is 3 mmol/L.The activities of peroxidase（POD） and polyphenoloxidase（PPO） were increased by oxalic acid and S.sclerotiorum induction to enhance disease resistance;the change of cellulase activity was increased at 96 h in inoculated leaves,the result showed that cellulase was related to their pathogenesis;the change of chitinase activity was increased at 72 h,chitinase was related to defense response.

关 键 词：油菜菌核病 多酚氧化酶 过氧化物酶 纤维素酶 几丁质酶 

分 类 号：TS201.2[轻工技术与工程—食品科学] Q55[轻工技术与工程—食品科学与工程]