血管生成抑制剂联合分化诱导剂治疗结肠癌肝脏转移的实验研究  

Experimental Study of the Therapy Effects of Angiogenesis Inhibitor and Differentiation Inducer on Human Colon Cancer

作　　者：邢会军[1] 崔秀成[1] 侯雷[1] 刘志满[1] 

机构地区：[1]承德医学院附属医院肿瘤一科,河北承德067000

出　　处：《河北医学》2011年第1期51-54,共4页Hebei Medicine

摘　　要：目的:实验通过建立人LOVO细胞结肠癌裸鼠肝脏转移瘤模型,研究血管生成抑制剂(TNP-470)联合分化诱导剂(RA)抗结肠癌转移的效应,二者是否具有协同效果。方法:建立人LO-VO细胞结肠癌裸鼠肝脏转移瘤模型,随机分为4组,模型建立后分组治疗,至治疗结束时处死裸鼠,检测肝脏转移率和肝转移结节数及肝脏转移瘤组织中血管内皮生长因子(VEGF)的表达。结果:结果显示,联合治疗组和TNP-470组与对照组及联合治疗组与RA组比较肝脏转移率均有显著性差异(P<0.05);联合治疗组和RA组与对照组比较,VEGF的表达量明显下降(P<0.05)。结论:血管生成抑制剂TNP-470与分化诱导剂RA联合治疗结肠癌裸鼠肝脏转移瘤比TNP-470或RA单药治疗具有更强的抑瘤作用,TNP-470通过抑制肿瘤血管生成,切断肿瘤细胞的血供和营养供给,抑制结肠癌裸鼠肝脏转移瘤的生长;而RA通过诱导肿瘤细胞凋亡,降低VEGF的表达抑制肿瘤的生长,二者联和应用,抗肿瘤转移效应明显增强。Objective： To study the anti-cancer metastasis effects of angiogenesis inhibitor TNP-470 combined with differention inducer RA by human colon cancer liver metastasis into nude mice.Method： Liver metastases tumor into nude mice with colon cancer of LOVO cell was established.The nude mice were divided into 4 groups at random.Treated respectively from one day after implanted and metastased,all the nude mice were sacrificed by the end of treatment,then observed the liver metastasis rate and tumor foci as well as expression of VEGF in the liver metastatic tumors were counted.Result： TNP-470 in combination with RA and TNP-470 alone display a significant inhibitory effect on liver metastasis compared to that of the control group（P0.05）,the combination group was different from RA group on liver metastasis（P0.05）.The expression of VEGF in the liver metastasis tumors was clearly inhibited by TNP-470 in combination with RA and RA alone compared to that of the control（P0.05）.Conclusion： The angiogenesis inhibitor TNP-470 in combination with differention inducer RA might has additive effect on the liver metastasis in nude mice.RA also could inhibit carcinoma cell proliferation by inducing apoptosis of colon cancer cell,inhibiting expression of VEGF.

关 键 词：结肠癌 血管生成抑制剂TNP-470 分化诱导剂RA 裸鼠模型 

分 类 号：R735.35[医药卫生—肿瘤]