磺氯酚N-Cu(Ⅱ)-HSA-OP体系对尿蛋白的测定  

Determination of Urine Protein by Sulfur Chlorophenol N-Cu(Ⅱ)-HSA-OP System

作　　者：胡秋娈[1] 冯爱青[2] 赵淑珍[1] 刘尚才[3] 

机构地区：[1]洛阳师范学院化学化工学院,河南洛阳471022 [2]洛阳师范学院生命科学系,河南洛阳471022 [3]洛阳正骨医院,河南洛阳471002

出　　处：《分析测试学报》2011年第1期68-71,共4页Journal of Instrumental Analysis

基　　金：河南省科技厅科技攻关重点项目(102102110154);河南省教育厅自然科学基金资助项目(200510482009)

摘　　要：室温下，在pH3．0的Britton—Robinson缓冲介质中，磺氯酚N与cu^2＋生成淡蓝色配合物，配合物与蛋白质发生反应形成多元深蓝色复合物，λmax为724nm，相比于磺氯酚N红移了174nm。初步探讨了反应机理，研究了反应体系的光谱性质及其影响因素，确定了最佳实验条件，建立了以多元反应为基础测定蛋白质的新方法。方法的ε724nm=3．06×105L·mol^-1·cm^-1，对HSA的检测线性范围为20～120mg／L。聚乙醇辛基苯基醚（0P）存在时，λmax基本不变，灵敏度提高31％，将该法用于尿蛋白含量的测定，结果满意。与双缩脲法相比，该方法的抗干扰能力强，灵敏度提高了16倍，适用于干扰较大的尿蛋白的测定。In pH 3.0 Britton -Robinson buffer, sulfur chlorophenol N bonded with Cu（ Ⅱ ） to form a blue complex, and the complex reacted with protein to form a dark blue multi-complex at room temperature which presents a maximum absorption at 724 nm and a red shift of 174 nm comparing with that of sulfur chlorophenol N. A new method for the determination of proteins was established based on the reaction of multi-compound substance. The spectral characterisitics of the system and its influ- encing factors were investigated. Under the optimal conditions, the apparent molar absorptivity and linear range for HSA were 3.06 × 10^5 L·mol^-1·cm^-1 and 20 - 120 mg·L-1 , respectively. With the presence of polyoxyethylene octyphenyl ether （ OP）, the sensitivity of the method could be increased by 31% . The mehtod was applied in the determination of urine protein with satisfactory results. And the sensitivity of the method is 16 times higher than that of the Biuret method.

关 键 词：光度法 磺氯酚N—Cu(Ⅱ)络合物 尿蛋白 

分 类 号：O657.32[理学—分析化学] O629.73[理学—化学]