韭菜有机溶剂提取液的气相色谱-质谱分析及其对香蕉枯萎病菌的抑制作用研究  

GC-MS Analysis of Solvent Extracts from Chinesechive and Their Inhibitory Action on Banana Vascular Wilt

作　　者：冯岩[1] 杨静美[1] 陈睿[2] 王晓容[3] 

机构地区：[1]仲恺农业工程学院农学院,广东广州510225 [2]仲恺农业工程学院化学化工学院,广东广州510225 [3]广东省农业科学院果树研究所,广东广州510640

出　　处：《分析测试学报》2011年第1期103-107,共5页Journal of Instrumental Analysis

基　　金：广东省重点科技资助项目(2007A020300002-9);广州市星火计划资助项目(2007C12E0061)

摘　　要：采用气相色谱-质谱法对韭菜茎和叶的石油醚、三氯甲烷、二氯甲烷、丙酮、乙酸乙酯有机溶剂提取液进行了检测。结果表明:有机溶剂提取液中有二甲基二硫、二甲基三硫、甲基甲烷硫代磺酸盐、二甲基酯硫磺酸、甲基2-丙烯基二硫及杂环等多种含硫化合物,另外还检出较复杂的3-乙烯基-1,2-二硫代杂环己-5-烯类物质。低温条件下,茎中石油醚、三氯甲烷、二氯甲烷、丙酮、乙酸乙酯的检出物分别为21、27、10、9、15种,叶中分别为14、6、9、3、9种;高温条件下,茎中石油醚、三氯甲烷、二氯甲烷、丙酮、乙酸乙酯的检出物分别为92、73、22、126、47种,叶中分别为29、18、未检出、24、9种。韭菜茎和叶不同部位及同一部位两种温度检出的主要化合物种类存在差别。韭菜茎的提取液所检出的化学物质多而复杂,高温条件下的检出物较低温的多。香蕉枯萎病菌孢子萌发实验结果表明,韭菜有机溶剂提取液中存在对香蕉枯萎病菌孢子有抑制作用的化学成分。The components extracted from Chinesechive by different solvents, such as petroleu- mether, chloroform, dichloromethane, acetone and ethylacetate were detected by GC - MS. The resuits showed that various sulfocompounds were obtained in solvent extracts, such as dimethyl disul- fide, dimethyl trisulfide, S-methyl methanethiosulfinate, sulfuric acid, dimethyl ester, disulfide, methyl 2-propenyl and heterocyclic, etc. Some rather complicated substances as 3-vinyl-1, 2-dithiacyclohex-5-ene, were also detected. At low temperature, the kinds of detected substances at stem and leaf extracted with petroleumether, chloroform, dichloromethane, acetone and ethylacetate were 21, 27, 10, 9, 15 and 14, 6, 9, 3, 9, respectively. At high temperature, the detected substances at stem and leaf were92, 73, 22, 126, 47 and29, 18, undetected, 24, 9, respectively. The kinds of main compounds at different parts of Chinesechive and different temperatures were different. The extractives from Chinesechive stem were excessive and complex. The compounds detected at high temperature were more than that at low temperature. The effect of solvent extractives on Fusarium oxysporum f sp. cubense spore was also investigated. The result indicated that some chemical compositions existed in solvent extractives from Chineseehive with inhibitory action on the germ.

关 键 词：韭菜 提取液 气相色谱-质谱 香蕉枯萎病菌 萌发 

分 类 号：O657.63[理学—分析化学] S435.673[理学—化学]