人胎盘源与人骨髓源间充质干细胞的生物学性状比较  

Biological characteristics of human placenta-derived mesenchymal stem cells versus human bone marrow-derived mesenchymal stem cells

作　　者：张红艳[1] 杨乃龙[1] 孙晓娟[1] 徐丽丽[1] 

机构地区：[1]青岛大学医学院附属医院特需保健科,山东省青岛市266003

出　　处：《中国组织工程研究与临床康复》2010年第45期8386-8389,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:人骨髓源间充质干细胞具有很好的临床应用价值,但数量和取材都有限,而人胎盘源间充质干细胞则相反,目前已成为再生医学的重要细胞来源和最具临床应用前景的功能干细胞。目的:比较人胎盘源间充质干细胞和人骨髓源间充质干细胞体外分离培养、扩增及生物学性状的差异。方法:采用胶原酶消化法分离人胎盘组织,密度梯度离心法分离骨髓单个核细胞,分别加入体积分数10%胎牛血清的LG-DMEM培养液,待细胞汇合至90%后消化传代。分别取第3代的人胎盘和骨髓间充质干细胞,按1×106浓度接种,当细胞达70%～80%融合时,换成成脂细胞诱导分化培养液,诱导16d。结果与结论:胎盘间充质干细胞、骨髓间充质干细胞均成贴壁生长、形态均一的成纤维样细胞梭形外观,但后者体积略小。两种细胞均高表达CD29、CD44,不表达CD34、CD106。二者均可分化为脂肪细胞。可见,从胎盘和骨髓中培养出的间充质干细胞在细胞形态、生长特性等方面基本相似,在体外均可有效扩增并成脂肪分化,均可作为组织工程的另一成体干细胞来源,而胎盘源间充质干细胞具有更好的应用前景。BACKGROUND：Human bone marrow-derived mesenchymal stem cells（BMSCs） have a great clinical value,but limited by the number and source,in contrast,human placenta-derived mesenchymal stem cells（PMSCs） have become an important cellular source in regenerative medicine and the stem cells of the most promising clinical application.OBJECTIVE：To compare the differences in isolation and culture in vitro,amplification and differences in biological characteristics between PMSCs and BMSCs.METHODS：Human placentas were isolated by collagenase and bone marrows were isolated by density gradient centrifugation.10% fetal calf serum was added to the LG-DMEM culture medium and cells were digested and passaged after converging to 90%.The third generation of PMSCs and BMSCs were vaccinated in the concentration of 1 × 106 and replaced by fat cells differentiated culture medium after converging to 70%-80% and induced for 16 days.RESULTS AND CONCLUSION：PMSCs and BMSCs had the adherent growth ability,morphology of fibroblast-like cells and spindle-shaped appearance,but the latter had the slightly smaller size.Both BMSCs and PMSCs highly expressed CD29,CD44,did not express CD34,CD106.Both could be differentiated into adipose cells.Cell morphology and growth characteristics are basically the same between BMSCs and PMSCs,both can amplify in vitro,differentiate into adipose cells and can be used as another source of adult stem cells in tissue engineering,but PMSCs have the better application prospects.

关 键 词：胎盘源间充质干细胞 骨髓源间充质干细胞 成脂细胞 诱导分化 差异 

分 类 号：R394.2[医药卫生—医学遗传学]