儿童骨髓增生异常综合征造血前体细胞增殖与白细胞介素15的影响  

Effects of interleukin-15 on proliferation of children's myelodysplastic syndrome hematopoietic stem/progenitor cells

作　　者：程涵蓉[1] 叶中绿[2] 陈铭珍[2] 

机构地区：[1]暨南大学第二临床医学院,深圳市人民医院儿科,广东省深圳市518020 [2]广东医学院附属医院儿科,广东省湛江市524023

出　　处：《中国组织工程研究与临床康复》2010年第45期8531-8534,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：湛江市科技攻关项目(020113),IL-15对儿童骨髓增生异常综合征造血干细胞增殖分化及凋亡的影响研究~~

摘　　要：背景:白细胞介素15是一种重要的骨髓生长调节因子,可能具有刺激骨髓增生异常综征CD34+细胞增殖的作用。目的:观察白细胞介素15对儿童骨髓增生异常综合征造血细胞增殖的影响。方法:采用细胞计数、MTT抑制实验检测0,10,20,50,100,200μg/L白细胞介素15对18例1～14岁儿童骨髓增生异常综合征患者CD34+细胞增殖的影响,找出白细胞介素15作用的峰浓度。以白细胞介素15达到峰值的浓度为实验组,不加白细胞介素15的为对照组,培养12d以了解细胞最佳增殖时间。结果与结论:体外培养的骨髓CD34+细胞在培养第8天生长状况最好,并且随着白细胞介素15质量浓度的增加,其活细胞数也随着增加,当白细胞介素15为100μg/L时,其增殖状况最好;当白细胞介素15为200μg/L时,其增殖状况反而欠佳。在培养的第8天,白细胞介素15对骨髓增生异常综合征患者骨髓CD34+细胞抑制率最低,当其浓度在0～100μg/L范围内,随质量浓度增加,其抑制作用递减,而当其质量浓度>100μg/L其抑制作用有所增强。说明白细胞介素15呈时间和剂量依赖性地促进骨髓增生异常综合征患者骨髓CD34+造血干/祖细胞增殖。BACKGROUND：Interleukin（IL）-15 is a member of hematopoietic cytokine superfamily.It may stimulate the proliferation of the myelodysplastic syndrome（MDS） hematopoietic stem/progenitor cells.OBJECTIVE：To investigate the effects of IL-15 on proliferation of hematopoietic cells in MDS child.METHODS：Cell-counting,MTT-inhibitory test were used to examine the inhibitory effect of IL-15（0,10,20,50,100,200 μg/L） on the proliferation of MDS CD34＋ cells in 18 children aged 1-14 years to find out the peak concentration of IL-15.IL-15 at peak concentration served as experimental group,whereas those without IL-15 as control group.12-day culture was done to understand optimal proliferation time of cells.RESULTS AND CONCLUSION：At day 8,CD34＋cells cultured in vitro grew well.With increased IL-15 concentration,living cells number increased as well.When IL-15 was 100 μg/L,its proliferation status was the best,but when IL-15 was 200 μg/L,its proliferation status was below the mark.At day 8,the inhibitory rate of IL-15 on CD34＋ cells was the lowest.When IL-15＇s concentration was between 0 and 100 μg/L.With increased IL-15＇s concentration,the inhibition decreased progressively.When IL-15 was above 100 μg/L,the inhibition reinforced on the contrary.These indicate that IL-15 can accelerate MDS bone marrow CD34＋ hematopoietic stem/progenitor cell proliferation in a dose-and time-dependent manner.

关 键 词：白细胞介素15 骨髓增生异常综合征 造血干细胞 增殖 CD34+细胞 

分 类 号：R394.2[医药卫生—医学遗传学]