人脂肪间充质干细胞的研究与应用(英文)  

Research and application of human adipose tissue-derived stromal cells

作　　者：孙建华[1] 刘杰[1] 赵国庆[1] 侯玲[1] 丁镌[1] 

机构地区：[1]黑龙江省血液中心输血研究所,黑龙江省哈尔滨市150056

出　　处：《中国组织工程研究与临床康复》2010年第45期8544-8550,共7页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:人脂肪间充质干细胞具有增殖和多向分化的能力,作为一种新型的干细胞已被广泛应用于组织工程研究,并在其已有的和潜在的生物治疗应用中发挥着重要作用。目的:通过检索相关文献,综述人脂肪间充质干细胞在组织工程和细胞治疗中的应用及存在的问题。方法:应用计算机检索CNKI和PubMed数据库中1960-01/2010-01关于人脂肪间充质干细胞应用的相关文章,检索词分别为"人脂肪间充质干细胞;分离;分化;免疫表型;应用"和"human adipose tissue-derived stromal cells;isolation;differentiation;immune phenotype;application",语言分别设定为中文和英文。选择内容与人脂肪间充质干细胞生物特性和应用相关的文章,同一领域文献则选择近期发表或发表在权威杂志的文章,排除重复类文章。结果与结论:收集到81篇相关文献,排除不符合标准的文献,共纳入57篇符合标准的文献。结果表明,人脂肪间充质干细胞具有许多特性,包括间充质干细胞潜在的增殖能力和适当条件下的多向分化能力。人脂肪间充质干细胞,不仅可以应用于组织的修复,而且在细胞免疫调节和基因治疗中均发挥重要作用。但人脂肪间充质干细胞的应用过程中仍存在许多问题。随着人脂肪间充质干细胞研究的深入,更多的生物学特性将会发现,人脂肪间充质干细胞在组织修复,细胞治疗,移植以及基因治疗中的应用前景会更加广阔。BACKGROUND：Human adipose tissue-derived stromal cells（ADSCs） possess exert proliferation and multi-directional ability.As a novel stem cell,it has been widely utilized in tissue engineering and plays an important role in biological and potential therapeutic applications.OBJECTIVE：To review the research progress,applications and existing problems of human ADSCs in tissue engineering and cell therapy by retrieving relevant publications.METHODS：PubMed and CNKI databases were undertaken to identify the relevant articles published from January 1960 to January 2009 with the Key words of ＂human adipose tissue-derived mesenchymal cells,isolation,differentiation,immune phenotype,application＂ both in English and Chinese.The articles relate to biological characteristics and applications of ADSCs were selected.In the same field,the documents published earlier or in the authoritative journals were preferred,and the repetitive studies were excluded. RESULTS AND CONCLUSION：A total of 81 documents were searched by computer,and 57 references were included in the final analysis.The results demonstrated that,human ADSCs share many characteristics,including the high proliferative potential and exhibiting the exert ability to undergo multilineage differentiation under appropriate conditions.Human ADSCs can not only utilize for tissue repairing,but also for cell immune modulation and gene therapy.However,there are still problems in its application.With the development of research on human ADSCs,their biological characteristics will be revealed,and their application in tissue repairing,cell therapy,transplantation,as well as gene therapy must be hold a great promise.

关 键 词：人脂肪间充质干细胞 分离 分化 免疫表型 组织工程 应用 

分 类 号：R394.2[医药卫生—医学遗传学]