Sphingomonas sp.X20絮凝特性及培养条件研究  

Sphingomonas sp.X20 Flocculating Characteristics and Study on Culture Conditions

作　　者：李锋[1] 杨小俊[1] 彭其安[1] 黄运平[1] 蔡亚君[1] 

机构地区：[1]武汉纺织大学,湖北武汉430073

出　　处：《生物技术》2010年第6期78-81,共4页Biotechnology

基　　金：国家"十一五"水体污染控制与治理科技重大专项子课题(2008ZX07317-002-01-05)资助

摘　　要：目的:对分离筛选获得絮凝剂产生菌Sphingomonas sp.X20絮凝特性及培养条件进行研究。方法:采用单因素和正交实验确定最适产絮凝剂培养条件。结果:研究发现,菌株X20的微生物絮凝剂对高岭土悬液具有良好的絮凝效果,在温度为37℃、培养基初始pH7.0、摇床转速为100r/min条件下培养12h获得的絮凝剂絮凝活性最好,絮凝率达92.8%。正交实验结果表明,菌株Sphingomonas sp.X20产絮凝剂最佳培养基的组成:淀粉15g/L,NH4Cl 1.0g/L,KH2PO42g/L,K2HPO45g/L,NaCl 0.1g/L,MgSO4.7H2O 0.2g/L,pH7.0。结论:菌株X20是一株高效的产絮凝剂菌,具有很好的应用前景。Objective： Study on flocculation characteristics and culture conditions of Sphingomonas sp.strain X20.Method：Optimum culture conditions were found by single factor and orthogonal experiments.Result：After cultured at 37℃,pH 7.0,100r/min for 12h,the culture of the strain X20 showed the best flocculation activity against Kaolin suspension,with the flocculating rate of 92.8%.The optimum component proportion of medium（g/L）of Sphingomonas sp.X20 which is Starch 15,NH4Cl 1.0,KH2PO4 2,K2HPO4 5,NaCl 0.1,MgSO4·7H2O 0.2,pH 7.0.Conclusion： Sphingomonas sp.strain X20 is an efficient flocculant producing bacteria which would have good prospects in application.

关 键 词：微生物絮凝剂 X20 高岭土 絮凝活性 正交实验 

分 类 号：X172[环境科学与工程—环境科学]