口腔鳞癌细胞系KB和Tca8113中的SP细胞含量分析  

Preliminary study on SP cells in oral squamous cell carcinoma cell lines KB and Tca-8113

作　　者：刘三霞[1] 鲁大鹏[1] 

机构地区：[1]首都医科大学口腔医学院,北京100050

出　　处：《北京口腔医学》2010年第6期329-331,共3页Beijing Journal of Stomatology

基　　金：北京市留学人员科技活动择优资助市优秀项目;北京市卫生局留学回国人员科研专项经费项目;教育部留学回国人员科研启动基金资(教外司留〔2004〕176号)

摘　　要：目的探讨两种口腔鳞状细胞癌细胞系KB和Tca8113中的侧群细胞的含量,寻找口腔鳞癌肿瘤干细胞存在的依据。方法采用有限稀释法对口腔鳞癌细胞系KB和Tca8113细胞株经Hoechst 33342/碘化丙啶(propidium iodide,PI)双染色后,采用流式细胞仪测定,分析两细胞株中侧群细胞的含量。结果口腔鳞癌细胞系KB和Tca8113中具有不同的表型,即侧群细胞和非侧群细胞。在体外培养中,KB和Tca8113细胞株中侧群细胞含量分别为0.6%和2.3%。经维拉帕米阻断后,侧群细胞含量均明显降低,分别降为0和0.1%。结论 KB和Tca8113细胞株中均存在侧群细胞,但含量较少。Objective To examine side population（SP）cells in oral squamous cell carcinoma cell lines KB and Tca-8113 in order to find the evidence that the tumor stem cells exists.Methods The SP cells in KB and Tca-8l13 cell lines of OSCC were detected by flow cytometry after Hoechst 33342/PI double staining.Results SP cells were found and accounted for about 0.6% of total KB cells,and about 2.3% of total Tca-8113 cells.Verapamil had the inhibitory effect on SP cells.Conclusion A small percentage of SP cells were found in KB and Tca-8113 cell lines.

关 键 词：口腔鳞状细胞癌细胞系 SP细胞 

分 类 号：R739.85[医药卫生—肿瘤]