银杏酸对口腔鳞癌多药耐药的影响  

The effect of ginkgolic acids on multidrug resistance in oral squamous cell carcinoma

作　　者：王辉[1] 周陈晨[1] 冯云[1] 戴丽娜[1] 陈娇[1] 陈思秀[1] 李小玉[1] 刘豫蓉[1] 张平[1] 

机构地区：[1]口腔疾病研究国家重点实验室,四川大学,四川成都610041

出　　处：《华西口腔医学杂志》2010年第6期668-671,共4页West China Journal of Stomatology

摘　　要：目的以卡铂(CBP)和平阳霉素(PYM)为诱导物,构建耐药细胞株Tca8113/CBP和Tca8113/PYM,再通过银杏酸(GAs)与化疗药物联用探讨银杏酸对耐药细胞多药耐药(MDR)的影响。方法免疫组织化学检测P-糖蛋白(P-gp)的表达,MTT法确定耐药细胞的耐药指数;不同质量浓度的GAs作用于耐药细胞及亲本细胞,通过MTT法检测对它们的增殖抑制效应,确定GAs的无细胞毒性质量浓度并观察GAs对耐药细胞的逆转作用;GAs与耐药细胞联用诱导细胞一段时间后,再次通过MTT法检测其耐药指数。结果免疫组织化学结果显示,耐药细胞的P-gp阳性表达率明显高于亲本细胞,MTT法确定GAs的无细胞毒性质量浓度为10μg.mL-1;GAs对Tca8113/CBP和Tca8113/PYM细胞的逆转倍数分别是2.94和2.43,与GAs联用前Tca8113/CBP和Tca8113/PYM细胞的耐药指数分别是3.24和11.9,联用后的耐药指数分别是2.18和4.43。结论本实验成功诱导出了耐药细胞株Tca8113/CBP和Tca8113/PYM,并将银杏酸与化疗药物联用,进一步证实了两者的共用能够增强对Tca8113/CBP和Tca8113/PYM细胞的增殖抑制作用,无细胞毒质量浓度的GAs对Tca8113/CBP和Tca8113/PYM细胞的耐药性有部分逆转作用,且共用一段时间后耐药细胞的MDR水平有所下降。Objective Carboplatin（CBP）-resistant cell line（Tca8113/CBP） and pingyangmycin（PYM）-resistant cell line（Tca8113/PYM） were established in vitro.Ginkgolic acids＇ influence over multidrug resistance（MDR） of drug-re-sistant cells was discussed by ginkgolic acids coupled with chemotherapy drugs.Methods The expression of P-glyco-protein（P-gp） was detected by immunohistochemistry.MTT assay was applied to ascertain the resistance index of drug-resistant cells.The effect of different concentrations of ginkgolic acids on the proliferation of drug-resistant cells and parental cell was measured by MTT assay.Making sure the non-toxic concentration of ginkgolic acids and ob-serving the reversal effect of ginkgolic acids on drug-resistant cells.Resistance index was redetermined by MTT as-say after ginkgolic acids coupled with chemotherapy drugs induced the cell lines for some time.Results Immunohistochemistry showed that P-gp positive expression rate of drug-resistant cells was significantly higher than parental cells.The non-toxic concentration of ginkgolic acids which was determined by MTT assay was 10 μg·mL-1.The reversal folds of Tca8113/CBP cell line to CBP and Tca8113/PYM cell line to PYM were 2.94 and 2.43 respectively. Before coupled with ginkgolic acids,the resistance indices of Tca8113/CBP and Tca8113/PYM cell lines were 3.24 and 11.9 respectively.When ginkgolic acids was added with chemotherapy drugs for some time,the resistance indices of Tca8113/CBP and Tca8113/PYM cell lines were 2.18 and 4.43 respectively.Conclusion This experiment successfully induced the drug-resistant cell lines of Tca8113/CBP and Tca8113/PYM.The method of chemotherapy drugs coupled with ginkgolic acids further confirmed the effect on proliferation of Tca8113/CBP and Tca8113/PYM cell lines was reducing.Non-toxic concentration of ginkgolic acids can partially reverse the drug resistance of Tca8113/CBP and Tca8113/PYM cell lines.Furthermore,MDR level of drug-resistant cells decreased somewhat when they were induced by

关 键 词：多药耐药 P-糖蛋白 银杏酸 

分 类 号：R739.8[医药卫生—肿瘤]