乙酰肝素酶对卵巢癌细胞侵袭和黏附的影响  

Heparanase promotes invasion and adhesion of ovarian carcinoma cells

作　　者：陈泓[1] 李力[2] 张玮[1] 王琪[1] 黎丹戎[1] 

机构地区：[1]广西医科大学第一附属医院妇产科,广西壮族自治区南宁530022 [2]广西医科大学附属肿瘤医院妇瘤科,广西壮族自治区南宁530021

出　　处：《中国肿瘤生物治疗杂志》2010年第6期639-643,共5页Chinese Journal of Cancer Biotherapy

基　　金：广西科技厅广西自然科学基金资助项目(No.2010GXNSFA013142)~~

摘　　要：目的:探讨乙酰肝素酶(heparanase,HPSE)在卵巢癌A2780细胞侵袭、转移中的作用。方法:构建携HPSE基因真核表达载体pcDNA3.1-HPSE,脂质体法将pcDNA3.1-HPSE和对照pcDNA3.1质粒转染至A2780细胞,G418筛选得稳定细胞株pcDNA3.1-HPSE-A2780和pcDNA3.1-A2780。MTT法和集落形成实验检测转染后A2780细胞的增殖;Matrigel侵袭、Transwell小室和黏附实验检测转染后A2780细胞的侵袭、迁移和黏附能力。结果:成功构建pcDNA3.1-HPSE载体,并转染入A2780细胞。pcDNA3.1-HPSE转染不影响A2780细胞的增殖(P>0.05),也不影响A2780细胞的迁移能力(P>0.05)。pcD-NA3.1-HPSE转染促进A2780细胞的侵袭(0.477±0.024vs0.250±0.081,P=0.003),降低其黏附能力(0.728±0.089vs0.518±0.080,P=0.002)。结论:HPSE通过促进肿瘤细胞的侵袭和降低黏附,在卵巢上皮癌浸润、转移中发挥重要作用。Objective：To explore the roles of heparanase （HPSE） in the invasion and metastasis of human ovarian carcinoma A2780 cells.Methods： pcDNA3.1-HPSE eukaryotic expression vector was constructed.pcDNA3.1-HPSE and empty pcDNA3.1 plasmids were transfected into A2780 cells by Lipofectamine method,and A2780 cells stably expressing pcDNA3.1-HPSE or pcDNA3.1 were selected by G418 （named pcDNA3.1-HPSE-A2780 or pcDNA3.1-A2780 cells）.The proliferation of A2780 cells after pcDNA3.1-HPSE transfection was detected by MTT and clone-forming experiment,and the invasion,migration and adhesion capacities of A2780 cells were tested by Matrigel,Transwell and Adhersion assays,respectively.Results： The pcDNA3.1-HPSE vector was successfully constructed and transfected into A2780 cells.pcDNA3.1-HPSE transfection had no effect on proliferation and migration of A2780 cells （all P0.05）.pcDNA3.1-HPSE transfection increased invasion （0.477±0.024 vs 0.250±0.081,P=0.003） and inhibited the adhesion of A2780 cells （0.728±0.089 vs 0.518±0.080,P=0.002）.Conclusion： Heparanase plays important roles in ovarian carcinoma by promoting invasion and inhibiting adhesion of tumor cells.

关 键 词：乙酰肝素酶 卵巢癌 侵袭 迁移 黏附 

分 类 号：R737.31[医药卫生—肿瘤] R730.2[医药卫生—临床医学]