The Arabidopsis PRCl-like ring-finger proteins are necessary for repression of embryonic traits during vegetative growth  

作　　者：Donghong Chen Anne Molitor Chunlin Liu Wen-Hui Shen 

机构地区：[1]Institut de Biologie Moleculaire des Plantes du CNRS, Universite de Strasbourg, 12 rue du General Zimmer, 67084 Strasbourg Cedex, France [2]Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization, Hunan Agricultural University, Changsha 410128, China

出　　处：《Cell Research》2010年第12期1332-1344,共13页细胞研究（英文版）

摘　　要：Polycomb group genes play crucial roles in the maintenance of the transcriptionally silenced state of genes for proper cell differentiation in animals and plants. While components of the polycomb repressive complex2 （PRC2） are evolutionarily conserved and their functions are extensively studied in plants, PRCI differs considerably between animals and plants, and its functions in plants are as yet not well described. Previous studies have identified the Arabidopsis AtRINGla and AtRINGlb as homologues of the animal PRC1 subunit RING1. Here, we show that the Atringla Atringlb double mutant exhibits derepression of embryonic traits during vegetative growth. Accordingly, several key regulatory genes involved in embryogenesis and stem cell activity are ectopically expressed in the mutant. Furthermore, we show that the mutant phenotypes and increased expression of regulatory genes are enhanced by the PRC2 mutant c/f. Finally, we show that three homologues of the animal PRCl-subunit ring-finger protein BMI1, AtBMIIa, AtBMIlb and AtBMIlc, can bind with AtRINGla or AtRINGIb, and in addition, AtBMIlc can bind with LHP1. The Atbmila Atbmilb double mutant shows derepression of embryonic traits similar to that of the Atringla Atringlb double mutant. Interestingly, expression levels of AtBMIla, AtBMIlb and AtBMIlc are elevated in the Atringla Atringlb mutant and those of AtBMIlc, AtRINGla and AtRINGlb are elevated in the Atbmila Atbmilb mu- tant, suggesting a self-regulatory feedback mechanism. Taken together, our results illuminate crucial functions of the PRCl-like ring-finger components in stable repression of embryonic traits and regulatory genes for proper somatic growth.

关 键 词：POLYCOMB PRC 1 RING 1 somatic embrvogenesis ARABIDOPSIS 

分 类 号：Q813[生物学—生物工程] S685.12[农业科学—观赏园艺]