MPTP参与硫化氢对大鼠心肌缺血再灌注损伤的延迟性保护作用  

MPTP involved the delayed protective effect of hydrogen sulfide on myocardial ischemic reperfusion injury in rats

作　　者：李双凤[1] 王丹[1] 王亚平[1] 冉珂[1] 唐正国 吕静[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,湖南长沙410011 [2]长沙市第三医院麻醉科,湖南长沙410011

出　　处：《东南大学学报（医学版）》2010年第6期648-651,共4页Journal of Southeast University(Medical Science Edition)

基　　金：湖南省卫生厅资助项目(B2009110)

摘　　要：目的:探讨硫化氢(H2S)预处理对大鼠心肌缺血再灌注损伤的延迟性保护作用及可能机制。方法:建立大鼠在体心肌缺血再灌注模型,设立假手术组(Sham组)、缺血再灌注组(IR组)、H2S预处理组(HS组)和线粒体通透性转换开孔(MPTP)开放剂苍术苷(ATR)+H2S组(HA组),每组8只。通过经颈动脉插管至左心室监测血流动力学,采用伊文思蓝和TTC染色检测心肌梗死面积。结果:缺血前,各组血流动力学参数差异无统计学意义(P>0.05)。缺血再灌注期间,与IR组比较,HS组各指标(HR、LVESP、±dp/dtmax)均升高,LVEDP降低,差异有统计学意义(P<0.05);与HA组比较,HS组各指标差异均无统计学意义(P>0.05)。与IR组比较,HS组心肌梗死范围明显减少(P<0.05),HA组无明显改变(P>0.05)。结论:硫化氢能改善大鼠心肌缺血再灌注损伤的心功能,这种心肌保护作用可能与MPTP的关闭有关。Objective： To investigate the delayed protective effect of hydrogen sulfide on myocardial ischemia/reperfusion（I/R） injury in rats and the possible mechanism.Methods： Thirty-two healthy Sprague-Dawley rats were randomly divided into 4 groups： sham group,IR group,hydrogen sulfide treatment group（HS group） and mitochondrial permeability transition pore（MPTP） opener atractyloside（ATR）＋hydrogen sulfide group（HA group）.We monitored hemodynamic parameters through carotid artery intubation and detected myocardial infarct size using Evans blue and TTC staining.Results： There were no significant differences in hemodynamic parameters between sham and model groups in preischemia condition.In the period of I/R,compared with IR group,HR,LVESP,and ＋ dp/dt max all increased and LVEDP decreased in HS group（all P0.05）,but there was no significant difference in HA group（P0.05）.Compared with IR group,the infarct size was reduced in HS group（P0.05）;there was no significant difference in HA group（P0.05）.Conclusion： Hydrogen sulfide has delayed protective effect against I/R injury in rat hearts,which may be related with inhibition of mitochondrial permeability transition pore opening.

关 键 词：硫化氢 缺血再灌注 线粒体通透性转换开孔 SPRAGUE-DAWLEY大鼠 

分 类 号：R-33[医药卫生] R649.9