Human herpesvirus 6A induces apoptosis of HSB-2 cellsvia a mitochondrion-related caspase pathway  

作　　者：Lingyun Li Jing Chi Feng Zhou Dandan Guo Fang Wang Genyan Liu Chun Zhang Kun Yao 

机构地区：[1]Department of Microbiology and Immunology,Nanjing Medical University,Nanjing,Jiangsu 210029,China [2]Department of Developmental Genetics,Nanjing Medical University,Nanjing,Jiangsu 210029,China [3]Department of Laboratory Medicine,the First Affiliated Hospital of Nanjing Medical University,Nanjing,Jiangsu 210029,China

出　　处：《The Journal of Biomedical Research》2010年第6期444-451,共8页生物医学研究杂志（英文版）

基　　金：supported by the National Natural Science Foundationof China (No. 30771961 and No. 30901344);Science Development Foundation of Nanjing Medical University (No. 08NMUZ003);Jiangsu Province Laboratory of Pathogen Biology (No. 08bykf01)

摘　　要：Apoptosis plays an important role in the pathogenesis of viral infections.In this study,we investigated the cell death processes during productive HHV-6A infection and the underlying mechanisms.Annexin V-PI staining and electron microscopy indicated that HHV-6A is a strong inducer of apoptosis.HHV-6A infection decreased mitochondrial transmembrane potential and led to morphological changes of mitochondria.The cell death was associated with activation of caspase-3 and cleavage of DNA repair enzyme poly （ADP-ribose） polymerase,which is known to be an important substrate for activated caspase-3.Caspase-9 was activated significantly in HHV-6A-infected cells,whereas caspase-8 was not activated obviously.Moreover,HHV-6A infection upregulated Bax and downregulated Bcl-2.This is the first demonstration of mitochondrion-mediated,caspase-dependent apoptosis in HHV-6A-infected cells.Apoptosis plays an important role in the pathogenesis of viral infections.In this study,we investigated the cell death processes during productive HHV-6A infection and the underlying mechanisms.Annexin V-PI staining and electron microscopy indicated that HHV-6A is a strong inducer of apoptosis.HHV-6A infection decreased mitochondrial transmembrane potential and led to morphological changes of mitochondria.The cell death was associated with activation of caspase-3 and cleavage of DNA repair enzyme poly （ADP-ribose） polymerase,which is known to be an important substrate for activated caspase-3.Caspase-9 was activated significantly in HHV-6A-infected cells,whereas caspase-8 was not activated obviously.Moreover,HHV-6A infection upregulated Bax and downregulated Bcl-2.This is the first demonstration of mitochondrion-mediated,caspase-dependent apoptosis in HHV-6A-infected cells.

关 键 词：human herpesvirus 6 APOPTOSIS CASPASE mitochondrion-mediated 

分 类 号：Q255[生物学—细胞生物学] S858.315.3[农业科学—临床兽医学]