腺病毒介导的nm23-H1对结直肠癌Lovo细胞作用的实验研究  

Adenovirus-mediated Delivery of nm23-H1 Gene Inhibits Growth of Colorectal Carcinoma Cell Line Lovo

作　　者：王琦[1,2] 何学令[2] 刘艳[2] 尹海林[2] 

机构地区：[1]四川大学生命科学学院,成都610064 [2]四川大学实验动物中心,成都610041

出　　处：《生物医学工程学杂志》2010年第6期1332-1335,共4页Journal of Biomedical Engineering

摘　　要：为研究重组腺病毒Ad-GFP-nm23-Hl抑制人结直肠癌Lovo细胞生长和转移的作用,采用MTT法及Tran-swell分别检测Ad-GFP-nm23-Hl对Lovo细胞体外增殖活性、黏附能力以及侵袭力的影响。结果显示,滴度为1010PFU/ml、109PFU/ml和108PFU/ml的Ad-GFP-nm23-H1对Lovo细胞的增殖抑制率分别为84.9%±1.51%、48.5%±7.23%和22.5%±5.47%,黏附抑制率分别为70.3%±2.40%、60.1%±5.68%和18.5%±3.61%,侵袭力抑制率分别为83.2%±5.71%、52.2%±6.94%和28.1%±8.21%。提示,Ad-GFP-nm23-Hl对人结直肠癌Lovo细胞的生长和转移具有抑制作用,为Ad-GFP-nm23-Hl进一步应用于肿瘤基因治疗提供了实验依据。This experimental study sought to find out the inhibitory effects of Ad-GFP-nm23-Hl on proliferation and metastasis of human colorectal carcinoma cell line Lovo,and,further,to gain an insight into some theoretical and methodical basis for instituting nm23-Hl gene therapy of cancers.MTT assay and Transwell chamber were used to detect the rates of proliferation and invasion as well as the adhesion of Lovo cells in vitro.The results demonstrated that the proliferation inhibition rates of Lovo cells treated with Ad-GFP-nm23-Hl of 1010 PFU/ml,109 PFU/ml and 108 PFU/ml were 84.9%±1.51%、48.5%±7.23% and 22.5%±5.47%,that the adherence inhibition rates of Lovo cells treated with Ad-GFP-nm23-Hl of 1010 PFU/ml,109 PFU/ml and 108 PFU/ml were 70.3%±2.40%,60.1%±5.68% and 18.5%±3.61%,and that the invasiveness inhibition rates of Lovo cells treated with Ad-GFP-nm23-Hl of 1010 PFU/ml,109 PFU/ml and 108 PFU/ml were 83.2%±5.71%,52.2%±6.94% and 28.1%±8.21%.These data suggested that Ad-GFP-nm23-Hl exerted significant inhibitory effects on the proliferation and metastasis of human colorectal carcinoma cell line Lovo in a dose-dependent way.

关 键 词：NM23-H1 人结直肠癌Lovo细胞 基因治疗 侵袭 

分 类 号：R735.3[医药卫生—肿瘤]