前列腺癌组织中间层上皮细胞比例差异的研究  

Proportion of intermediate epithelial cells and human prostate cancer

作　　者：于殿君[1] 唐悦清[2] 施云峰[1] 王永传[1] 卓见[1] 朱轶勇[1] 孙晓文[1] 夏术阶[1] 

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,上海200080 [2]山东大学齐鲁医院泌尿外科,山东济南250012

出　　处：《中华男科学杂志》2010年第12期1063-1067,共5页National Journal of Andrology

基　　金：国家自然科学基金(30600618);上海市科委项目(07QA14037)~~

摘　　要：目的:探讨前列腺癌组织中间层上皮细胞构成比例差异及其临床意义。方法:对60例前列腺癌标本分别进行CK5、CK8免疫组化染色将前列腺癌分为两类:即以中间层上皮细胞为主的前列腺癌(CaP-INT)共计32例和以腔面层上皮细胞为主的前列腺癌(CaP-LUM)共计28例。比较两类前列腺癌雄激素受体(AR)阳性率、患者年龄、血清t-PSA、前列腺体积、G leason评分、临床分期、雄激素抵抗率的差异。结果:两类前列腺癌相比较,CaP-LUM的AR阳性率为(77.21±10.22)%,而CaP-INT其阳性率为(24.42±11.41)%,CaP-INT的AR阳性率明显低于CaP-LUM(P<0.05)。远处转移的病例数,CaP-INT为14例,CaP-LUM为4例,CaP-INT转移率明显高于CaP-LUM(P<0.05)。在行内分泌治疗的患者中,CaP-INT组共26例,有6例进展为雄激素非依赖性前列腺癌(AIPC);CaP-LUM共23例,有1例进展为AIPC,两者亦有显著差异(P<0.05)。CaP-INT在局部临床分期上也显著高于CaP-LUM(P<0.05)。而两类前列腺癌在年龄、血清t-PSA、前列腺体积及G leason评分上无显著差异(P>0.05)。结论:中间层上皮细胞增多和前列腺癌侵袭性增加、肿瘤转移性增强及产生雄激素抵抗相关。Objective：To study the different proportions of intermediate epithelial cells in human prostate cancer tissue and their clinical significance.Methods：We performed immunohistochemical staining for Cytokeration 5（CK5） and Cytokeration 8（CK8） on 60 samples of human prostate cancer,determined the proportions of intermediate epithelial cells in the cancer tissue,and classified the samples into 2 types,one with a majority of intermediate epithelial cells（CaP-INT,n = 32）,and the other composed mostly of luminal epithelial cells（CaP-LUM,n = 28）.Then we compared the 2 types of prostate cancer in the expression of the androgen receptor（AR）,age of the patient,serum t-PSA,prostate volume,Gleason score,clinical stage,androgen resistance,and incidence of distant metastasis.Results：CaP-INT showed a significantly lower expression of AR（[24.42 ± 11.41] %） and a higher incidence of distant metastasis（n = 14） than CaP-LUM（[77.21 ± 10.22] % and n = 4）（P〈0.05）.In the CaP-INT group,6 of the 26 endocrinologically treated cases developed into androgen-independent prostate cancer（AIPC）,while in the CaP-LUM group,only 1 out of 23（P〈0.05）.The former also showed remarkably higher clinical stages than the latter（P〈0.05）,but no significant differences were found in age,serum t-PSA,prostate volume and Gleason score between the two groups（P〈0.05）.Conclusion：A higher proportion of intermediate epithelial cells may lead to increased invasiveness and metastasis of human prostate cancer.

关 键 词：前列腺癌 雄激素受体 CK5 CK8 中间层上皮细胞 雄激素抵抗 

分 类 号：R737.25[医药卫生—肿瘤]