响应面分析法优化微波提取夏枯草中黄酮化合物的工艺研究  

Optimization of Microwave-Assisted Extraction of Flavonoids from Prunella vulgaris L.Using Response Surface Methodology

作　　者：张国文[1] 胡明明[1] 何力[1] 潘军辉[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,江西南昌330047

出　　处：《南昌大学学报（工科版）》2010年第4期385-389,共5页Journal of Nanchang University(Engineering & Technology)

基　　金：江西省自然科学基金资助项目(2009GZH0069);南昌大学食品科学与技术国家重点实验室资助项目(SKLF-TS-200917;SKLF-MB-200807)

摘　　要：研究了响应曲面法微波辅助提取夏枯草中黄酮化合物的工艺。在单因素试验的基础上,利用Box-Benhnken中心组合试验和响应面分析法,研究乙醇体积分数(V/V)、液固比(mL/g)和提取时间(s)等因素对黄酮类化合物得率的影响。结果表明,微波提取夏枯草中黄酮类化合物的最佳工艺条件:乙醇体积分数20%、液固比24:1、提取时间116 s,黄酮化合物的实测结果(3.85%)与响应曲面拟合所得方程的预测值(3.87%)符合良好。The microwave-assisted extraction（MAE） process of flavonoids from Prunella vulgaris L.was studied.On the basis of single-factor test,the effects of ethanol concentration,ratio of liquid to solid,extraction time on yield were investigated through Box-Benhnken center composite design and response surface methodology（RSM）.The optimum conditions of MAE of flavonoids from Prunella vulgaris L.can be concluded as follows：ethanol concentration 20%（V/V）,ratio of liquid to solid 24：1 mL/g,extracting time 116 s.Under these conditions,the practical yield of flavonoids was 3.85%,which was consistent with the predicted yield of flavonoids of 3.87%.

关 键 词：响应面分析法 夏枯草 黄酮 微波提取 

分 类 号：TS207.3[轻工技术与工程—食品科学]