5-氟尿嘧啶联合热疗对人肝癌细胞株的增殖抑制作用  

Inhibition of proliferation of 5-FU combined with thermotherapy in human hepatoma cell line

作　　者：刘贤英[1] 徐茂凤 金春香[5] 张巍[3] 李香俊[4] 杨慧慧[5] 孙寒[1] 

机构地区：[1]吉林大学第二医院,长春130041 [2]长春医学高等专科学校 [3]吉林大学护理学院 [4]延边大学医学院 [5]吉林大学第一医院

出　　处：《中国综合临床》2010年第12期1233-1235,共3页Clinical Medicine of China

摘　　要：目的 探讨5-氟尿嘧啶（5-FU）联合热疗诱导人肝癌细胞株（SSMC7721细胞）的增殖抑制率,细胞凋亡率及其对细胞周期进程的影响,旨在为肝细胞癌的综合治疗提供理论依据.方法 应用MTT比色法检测不同条件下对SSMC7721细胞的增殖抑制率,流式细胞仪检测细胞周期进程的变化及凋亡率,电子显微镜检测亚细胞结构改变.结果 单纯热疗组、5-FU组及联合组对SSMC7721细胞抑制率分别为18.4%、28.3%和52.7%,与对照组相比差异均有统计学意义（P均＜0.01）.流式细胞仪结果显示：G1期细胞增多,S期细胞减少、G2/M期细胞相对增多,细胞凋亡率升高,组间比较差异均有统计学意义（P＜0.01或P＜0.05）,电子显微镜结果显示细胞核染色质趋边凝集、线粒体肿胀.结论 5-FU联合热疗能显著提高SSMC7721细胞的增殖抑制率,抑制SSMC7721细胞G1期向S期转化进程,诱导SSMC7721细胞凋亡及亚细胞结构改变.Objective To investigate the inhibition rate of cell proliferation, cell apoptosis rate and their effects on the cell cycle proceeding of the SSMC7721 cell line when 5-FU combined with thermotherapy is induced into the cells, and then provide theoretical bases to the combined therapy of hepatocellular carcinoma. Methods The inhibition rate of cell proliferation was detected by the MTT under different conditions, the cell cycle proceeding and the cell apoptosis rate was detected by flow cytometry and the subcellular structure was detected by the electronmicroscope. Results The cell inhibition rate of the thermotherapy group, 5-FU group and the combinedgroup were 18.4% ,28. 3% and 52. 7% ,respectively. The inhibition rates in the latter two groups were significantly different to the thermotherapy group. The results of flow cytometry showed that the cell numbers increased in G1 stage decreased in S stage,and increased in G2/M stage;the cell apoptosis rate increased. There was significant difference between difference between different groups （ P 〈 0. 01 or P 〈 0. 05 ）. The results of the electronmicroscop showed that the nuclear chromatins agglutinated in the borderline and the mitochondriums became swelled. Conclusions The 5-FU combined with thermotherapy could significantly improve the inhibition rate of cell proliferation,inhibit the cell cycle proceeding from G1 stage to S stage, and induce cells apoptosis and change the subcellular structures in the SSMC7721 cell line.

关 键 词：人肝癌细胞株 5-氟尿嘧啶 热疗 细胞周期 

分 类 号：R735.7[医药卫生—肿瘤]