贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法研究  

A Tricine-SDS-PAGE Electrophoresis Method for Determination of Kinase Components in Guizhou Douchi

作　　者：高秀丽[1] 宋智魁[1] 王清忱[2] 孟小夏[1] 舒阳[1] 张敏[1] 王鹏娇[1] 

机构地区：[1]贵阳医学院药学院,贵州贵阳550004 [2]贵州省人民医院,贵州贵阳550004

出　　处：《贵阳医学院学报》2010年第6期575-577,共3页Journal of Guiyang Medical College

基　　金：贵阳市科技局[(2009)筑科农合同字第2-009号]

摘　　要：目的:建立一种能够稳定检测贵州豆豉激酶组分的Tricine-SDS-PAGE电泳方法。方法:采用Tricine-SDS-PAGE电泳方法,分离胶浓度为12%,交联度为3%,以blue-silver染色法进行染色,对贵州豆豉激酶组分进行分析。结果:贵州豆豉激酶组分经Tricine-SDS-PAGE电泳后,在蛋白Marker在20.1～43 kDa之间,出现5条较清晰的条带。结论:Tricine-SDS-PAGE电泳方法稳定可靠,能够用于贵州豆豉激酶组分的检测。Objective： To establish a tricine-SDS-PAGE method for stable determination of kinase components in Guizhou douchi.Method： Tricine-SDS-PAGE was used,of which the gel concentration and cross-linking density were 12% and 3%,respectively.After electrophoresis,the gel was stained with ＂blue-silver＂ method.Results： Five bands between 20.14 kd and 43 kd were found on the electrophoretogram.Conclusion： Tricine-SDS-PAGE could detect the components of Guizhou douchi steadily and reliably.

关 键 词：电泳 贵州豆豉激酶组分 纤溶酶 发酵 

分 类 号：R446.11[医药卫生—诊断学]