从福尔马林固定的癌组织中提取RNA的方法  

A Method for RNA Extraction from Formalin-fixed Cancer Tissue

作　　者：邓昊[1,2] 靳楠[1] 张磊[1] 刘丽江[1,2] 

机构地区：[1]江汉大学医学院病理学与病理生理学教研室,湖北武汉430056 [2]江大病理诊断所,湖北武汉430056

出　　处：《江汉大学学报（自然科学版）》2010年第4期85-89,共5页Journal of Jianghan University：Natural Science Edition

基　　金：国家自然科学基金面上项目(30870981);湖北省自然科学基金(2006AB191);武汉市青年科技晨光计划项目(20045006071-7)

摘　　要：目的：寻求从福尔马林固定的癌组织中提取RNA的合理方法.方法：运用Trizol法从福尔马林固定的胃癌组织中提取RNA,用分光光度计测定RNA的产量和纯度;通过RT-PCR对不同长度的管家基因-actin进行扩增.结果：100 bp和300 bp的-actin均能顺利扩增出特异性条带,500 bp的-actin未能扩增.结论：用Trizol法从福尔马林固定的癌组织中提取RNA是可行的;进行RT-PCR时应尽量设计小片段引物（100~300 bp）以获得更好的扩增效果.Objective：To find a reasonable method for RNA extraction from formalin-fixed cancer tissue.Methods：To extract RNA from the formalin-fixed gastric cancer tissue with Trizol method.RNA yield and purity were detected with spectrophotometer,and RNA quality were de-tected by RT-PCR of different length housekeeping gene-actin.Results：High purity RNA can be extracted from the formalin-fixed gastric cancer tissue with this method.RT-PCR results showed that the 100 bp and 300 bp-actin can be successfully amplified,and the 500 bp-actin failed to am-plify specific bands.Conclusion：With Trizol method extracting RNA from formalin-fixed cancer tissue is feasible.We should try to design the small fragments primers（100-300bp） in order to obtain a higher amplification efficiency while carrying on RT-PCR.

关 键 词：癌组织 福尔马林 固定 RT-PCR TRIZOL法 

分 类 号：R730.21[医药卫生—肿瘤]