毕赤酵母发酵生产T4溶菌酶的中试研究  

The Pilot Study on T4 Lysozyme Fermentation by Pichia pastoris

作　　者：吴家鑫 余志强 王华丽 葛辛玫 齐鹏 郑应华 刘德虎[2] 宋敏 张国栋 曹芹 

机构地区：[1]中牧实业股份有限公司兽药研究所,北京100091 [2]中国农业科学院生物技术研究所,北京100081

出　　处：《中国生物工程杂志》2010年第12期49-52,共4页China Biotechnology

基　　金："十一五"国家科技支撑计划(2006BAD31B01-04)资助项目

摘　　要：研究了T4溶菌酶的中试发酵放大生产。在200L发酵罐中毕赤酵母诱导表达T4溶菌酶蛋白,发酵液蛋白表达量达到1.69g/L,酶活达到192U/mg;制得冻干酶粉211.1g。经测定粗酶粉中T4溶菌酶的含量达到68.9%,酶活为182U/mg,总收率达到66%,成功建立了一条T4溶菌酶的中试发酵生产工艺线路。T4 lysozyme pilot-scale fermentation was studied.T4 lysozyme was induced and expressed by Pichia pastoris in the 200L fermentor.The yield of target protein was 1.69g /L with 192U/mg enzyme activity in the broth.The enzyme protein was freeze-dried.The weight of enzyme powder was 211.1g.T4 lysozyme content was 68.9%,and activity was 182U/mg in enzyme powder.The total yield was 66%.The T4 lysozyme pilot-scale process line was established successfully.

关 键 词：T4溶菌酶 中试 毕赤酵母 

分 类 号：Q819[生物学—生物工程]