细胞角蛋白19联合人乳头状瘤病毒DNA(16/18型)检测早期宫颈癌淋巴结微转移  

Cytokeratin 19,human papillomavirus combined DNA(16/18 type) early detection of UCC lymph node micrometastasis

作　　者：柴凤霞[1] 樊玉兰[1] 窦心灵[1] 

机构地区：[1]甘肃省酒泉市人民医院,735000

出　　处：《国际检验医学杂志》2010年第12期1394-1395,1397,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨早期宫颈癌患者淋巴结中细胞角蛋白19(CK-19)mRNA和人乳头状瘤病毒(HPV)DNA(16/18型)的表达及联合检测的临床意义。方法采用逆转录-聚合酶链反应(RT-PCR)技术、荧光定量-聚合酶链反应(FQ-PCR)技术,检测CK-19mRNA和HPVDNA(16/18型)在30例早期宫颈癌患者194枚淋巴结中的阳性率及二者联合检测的敏感度和准确度。结果 30例早期宫颈癌患者的淋巴结194枚,常规病理学HE染色检出8例患者26枚淋巴结转移、22例患者无转移淋巴结164枚。在22例HE染色未检出淋巴结转移组中,聚合酶链反应检测9例患者40枚淋巴结CK-19mRNA呈阳性,12例患者68枚淋巴结HPVDNA(16/18型)呈阳性。转移淋巴结组和无转移淋巴结组CK-19mRNA阳性率分别为92.3和23.8,HPVDNA(16/18型)阳性率分别为96.2和40.5;比较两组淋巴结CK-19mRNA和HPVDNA(16/18型)阳性检出率,转移淋巴结组明显高于无转移淋巴结组(P<0.01)。单独检测CK-19mRNA敏感度为33.0,HPVDNA敏感度为47.9;采用二者联合检测,敏感度可达76.8,准确度为81.8。结论 CK-19mRNA和HPVDNA(16/18型)可作为判断早期宫颈癌淋巴结微转移的参考指标,二者联合检测有较高的检出率。Objective To study the expression of cytokeratin 19 （CK 19） mRNA and human papilloma virus （HPV） DNA （16/18 type） in lymph nodes of patients with early stage uterine cervix cancer （UCC）, and to explore the clinical significance of combined detection. Methods Reverse transcription-polymerase chain reaction （RT-PCR） technology, quantitative-polymerase chain reaction （FQ-PCR） technique had been used to detect CK-19 mRNA and HPV DNA （16/18 type） in 194 lymph nodes from 30 patients with early stage UCC,including positive rate and sensitivity and accuracy of combination detection. Results In 194 lymph nodes,there were 26 lymph holies from 8 patients with metastasis,and 164 lymph nodes from 22 patients without metastatic detected by routine HE staining. In non metastatic group, there were 40 lymph nodes from 9 patients positive for CK-19 mRNA, and 68 lymph nodes from 12 patients positive for HPV DNA （16/18 type） ,which were detected by RT-PCR. The positive rates of CK- 19 mRNA of metastatic group and non metastatic group were 92.3% and 23.8% ,respectively,and the positive rates of HPV DNA （16/18 type） were 96.2% and 40.5 % ,respectively. Compared with non metastatic group, the positive rates of CK-19 mRNA and HPV DNA （16/18 type） were significantly higher （P〈0.01）. The sensitivity of CK-19 mRNA individual detection was 33.0%, and 47.9% was for HPV DNA （16/18 type）. The sensitivity and accuracy were up to 76.8% and 81.8% respectively in combined detection. Conclusion CK-19 mRNA and HPV DNA （16/18 type） could be used as UCC lymph node micrometastasis in early reference. The combined detection had a higher detection rate.

关 键 词：角蛋白质类 人乳头瘤病毒16 人乳头瘤病毒18 DNA 宫颈肿瘤 淋巴结 肿瘤转移 mRNA信使 

分 类 号：R737.33[医药卫生—肿瘤]