MIB-1表达与子宫颈鳞状细胞癌局部微血管微淋巴管密度及浸润转移和预后的关系  

Relationship between Expression of MIB-1 Protein and Regional Microvessel Density, Lymphatic Microvessel Density, Invasion, Metastasis and Prognosis in Squamous Carcinoma of Cervix

作　　者：齐碧如[1] 江忠清[2] 连成瑛[2] 黄免[1] 林岷 陈玉华[1] 林玲[2] 钟莉娉[2] 

机构地区：[1]福建省福州市第一医院妇产科,350009 [2]福建医科大学附属第一医院妇科,福州350005 [3]福建医科大学基础医学院生理学与病理生理学系,350004

出　　处：《中国医疗前沿》2010年第21期15-18,共4页China Healthcare Innovation

基　　金：福建省教育厅项目(编号:JA04210;JA03104);福州市科技局项目(编号:2007-S-124)

摘　　要：目的探讨MIB-1在子宫颈鳞癌的表达及临床意义。方法应用免疫组化技术检测28例正常子宫颈上皮(NCE),36例子宫颈上皮内瘤变(CIN)和68例子宫颈鳞癌(SCC)组织增殖指数(PI,MIB-1标记)、微血管密度(MVD,CD34标记)和微淋巴管密度(LMVD,VEGFR-3标记)。结果 MIB-1阳性染色定位于肿瘤细胞核。MIB-1标记的PI在NCE、CIN及SCC组分别为96.0±54.6、300.0±157.1和378.7±199.6。从NCE到CIN再到SCC组,PI显著升高(P<0.05)。MIB-1在CIN及SCC组表达均分别与MVD显著正相关(CIN:r=0.442,P=0.007;SCC:r=0.253,P=0.037),而与LMVD无显著相关性(CIN:r=0.099,P=0.567;SCC:r=0.159,P=0.196)。MIB-1在SCC组表达分别与有否盆腔淋巴结转移、脉管浸润、间质浸润深度及生存率有关(P<0.05)。有盆腔淋巴结转移、脉管浸润及突破深肌层间质浸润者,其PI均分别显著高于无盆腔淋巴结转移、无脉管浸润及未突破深肌层间质浸润者(P<0.05)。MIB-1高表达者生存率显著低于低表达者(P=0.0383)。结论 MIB-1可能在子宫颈鳞癌发生和浸润转移过程中发挥重要作用,可能是子宫颈鳞癌患者不良预后的标记物。Objective The Expression and clinical significance of MIB-1 in squamous cell carcinoma of cervix was investigated. Method The proliferation index（PI） labeled by MIB-1, microvessel density（MVD） labeled by CD34 and lymphatic microvessel density（LMVD） labeled by VEGFR-3 protein in 28 cases of normal cervical epithelium（NCE）, 36 cases of cervical intraepithelial neoplasm（CIN） and 68 cases of squamous cell carcinoma of cervix（SCC） were detected by immunohistochemistry SP method. Results The staining reaction of MIB-1 was mainly confined to the cellular nucleus in cervical neoplasm. The PI labeled by MIB-1 in NCE, CIN and SCC groups was 96.0±54.6, 300.0±157.1 and 378.7±199.6, respectively. The PI increased remarkably from NCE to CIN and then to SCC accordingly（P0.05）. The expression of MIB-1 in CIN and SCC groups was positively correlated with MVD（for CIN： r=0.442, P=0.007; for SCC： r=0.253, P=0.037）, but not correlated with LMVD（for CIN：r=0.099, P=0.567; for SCC： r=0.159, P=0.196）, respectively. In SCC, the expression of MIB-1 correlated with pelvic lymph node metastasis, intravascular infiltration, stroma involvement and survival rate（P0.05）. In the cases with pelvic lymph node metastasis, intravascular infiltration and deeper stroma involvement, the PI was significantly higher than that in those without the conditions mentioned above（P0.05）. The patients with higher expression of MIB-1 in SCC group had significantly lower survival rate than that in those with the lower MIB-1（P=0.0383）. Conclusion MIB-1 may play an important role during the canceration, cancer invasion and matastasis, and may be a marker of bad prognosis in squamous carcinoma of cervix.

关 键 词：宫颈肿瘤 MIB-1 微血管密度 微淋巴管密度 肿瘤转移 预后 免疫组织化学 

分 类 号：R737.33[医药卫生—肿瘤]