湖北省长角血蜱携带嗜吞噬细胞无形体的调查  

Investigation on the Haemaphysalis longicornis infected with Anaplasma phagocytophilum in Hubei Province,China

作　　者：张令要[1] 李静[1] 詹发先[1] 袁方玉[1] 徐军强[1] 岳金亮[1] 谭良飞[1] 詹建波[1] 尹华超[2] 王本银[2] 朱建华[2] 崔雪峰 

机构地区：[1]湖北省疾病预防控制中心,武汉430079 [2]随州市疾病预防控制中心,随州441300 [3]兴山县疾病预防控制中心,兴山443100

出　　处：《中国人兽共患病学报》2010年第12期1148-1150,共3页Chinese Journal of Zoonoses

基　　金：2008-2010年度湖北省卫生厅青年科技人才基金项目

摘　　要：目的调查湖北省长角血蜱是否携带嗜吞噬细胞无形体。方法运用聚合酶链反应方法对湖北随州地区采集的蜱标本进行嗜吞噬细胞无形体16SrRNA基因片段进行扩增和序列分析,将扩增序列与GenBank注册的基因序列进行比较。结果共检测游离蜱72只,蜱种鉴定均为长角血蜱,嗜吞噬细胞无形体最小感染率为4.22%;所检出的嗜吞噬细胞无形体16SrRNA基因与我国已在GenBank注册的24个相对应序列同源性100%。结论湖北省长角血蜱携带嗜吞噬细胞无形体。To investigate the prevalence of the Anaplasma phagocytophilum in ticks in Hubei Province,16S rRNA of A.phagocytophilum collected from ticks were amplified with PCR and sequence analysis.A total of 72 Haemaphysalis longicornis were tested,and the minimum infection rate was 4.22%.The sequence of 16S rRNA amplified was compared with the corresponding parts of the sequence deposited in GenBank,the similarities of the nucleotide sequences with 24 genes deposited in GenBank were 100%.It is concluded that the infection of A.phagocytophilum in H.longicornis is demonstrated for the first time in Hubei Province,China.

关 键 词：嗜吞噬细胞无形体 16SRRNA基因 长角血蜱 

分 类 号：R384.4[医药卫生—医学寄生虫学]