ABCC4表达与胃癌细胞增殖的相关性研究  

The relationship between ABCC4 expression and gastric cancer cell proliferation

作　　者：佟柏峰[1] 李云龙[1] 宋茂力[1] 贾洪波[1] 巴图 李杰 

机构地区：[1]哈尔滨医科大学附属第二临床医学院普外科,150081 [2]内蒙古牙克石市博克图医院外科,022181

出　　处：《国际遗传学杂志》2010年第6期326-329,363,共5页International Journal of Genetics

基　　金：黑龙江省卫生厅科研课题资助（2010-069）

摘　　要：目的 观察ABCC4（ATP-binding cassette,sub-family C member4）基因对胃癌细胞系SGC-7901增殖及凋亡的作用.方法 在胃癌细胞系SGC-7901中导入携带ABCC4的RNAi慢病毒载体,应用CELLOMICS检测细胞生长及克隆形成情况,采用流式细胞术检测细胞周期及细胞凋亡情况.结果 SGC-7901细胞经ABCC4基因RNAi有效靶点慢病毒（ABCC4 siRNA）感染后,SGC～7901细胞生长减慢,细胞周期发生改变,处于G1期的SGC7901细胞显著减少,（t=2.752,P＜0.05）,处于G2/M期的SGC-7901细胞显著增加（t=2.973,P＜0.05）,同时,细胞凋亡显著增加（t=3.068,P＜0.05）,细胞克隆形成能力减弱（t=3.534,P＜0.05）.结论 抑制ABCC4基因可以减缓SGC-7901细胞恶性增殖,促进凋亡.Objective To observe the effect of ABCC4（ATP-binding cassette, sub-family C member 4） on gastric cancer cell line SGC-7901＇s proliferation and apoptosis. Methods SGC-7901 cell line were infected by the lentivirus carried the RNAi of ABCC4. were detected the proliferation and clone formation through CELLOMICS. Flow cytometry was applied to detect the cell cycle and apoptosis. Results The infection of SC C-7901 cell line with lentivirus carried the RNAi of ABCC4 alleviated the proliferation of cell line, changed the cell cycle, significantly decreased the cell number of Go/G1 （t=2.752,P〈0.05）, significantly increased the cell number of G2/M （t=2.973, P〈0.05）, at the same time significantly increased apoptosis （t= 3.068, P〈0.05）, and inhibited the formation of cell clone （t = 3.534, P〈0.05）. Conclusion The inhibition of gene ABCC4 can alleviate the malignant proliferation of SGC-7901 and produce its apoptosis.

关 键 词：ABCC4 胃癌 细胞增殖 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]