利用PCR-DGGE技术鉴定硝基甲苯类物质降解菌株  

Determination on High Effective Strains of Degrading Nitrotoluene Using PCR-DGGE

作　　者：尹艳华[1] 胡学敏[1] 付民[1] 

机构地区：[1]北京理工大学化工与环境学院,北京100081

出　　处：《北京理工大学学报》2011年第1期100-104,共5页Transactions of Beijing Institute of Technology

摘　　要：为鉴定光催化-厌氧好氧固定化微生物方法降解2,6-DNT和4-MNT的优势菌株,取连续运行40d的厌氧好氧反应器出入口的微生物样品.采用PCR-DGGE技术分离微生物,得到对2,6-DNT和4-MNT具有良好降解特性的优势菌种;发现厌氧反应器内不同位置微生物种类差别很大,好氧反应器内差别很小;反应器内有5种优势菌株:Delta proteobacterium,Pseudomonas stutzeri,Pseudomonas trivialis,Burkholderia cenocepa-ciaand Chryseobacterium sp.AKB-2008-VA6和1个未知菌株,以兼性厌氧和好氧方式生存.反应器运行期间,水质指标良好.To identify the dominant degrading 2, 6-dinitrotoluene （2, 6-DNT） and 4- mononitrotoluene（4-MNT） bacteria in the photocatalysis-anaerobic-aerobic progress, the microbe samples were collected individually from the inlet and outlet of 40 days running anaerobic-aerobic reactor. The measures of polymerase chain reaction （PCR） and denaturing gradient gel electrophoresis （DGGE） were cooperatively used to segregate microbes in the photocatalysisanaerobic-aerobic microbial community for obtaining the dominant efficiently degrading 2, 6- dinitrotoluene and 4-mononitrotoluene bacteria. It is found that the difference of microbe types is large at different locations in anaerobic reactor, but is less in aerobic reactor. There are five dominant microbes： Delta proteobacterium , Pseudomonas stutzeri , Pseudornonas trivialis , Burkholderia cenocepaciaand Chryseobacterium sp. AKB- 2008-VA 6 and one unknown microbes. The dominant microbes are facultative anaerobic-aerobic. The water quality is good in 40 days reactor operation.

关 键 词：硝基甲苯类物质 优势菌种 聚合酶链式反应 变性梯度凝胶电泳 

分 类 号：X52[环境科学与工程—环境工程]