小鼠急性乙醇性胃黏膜损伤模型的制备  

Establishment of animal model of ethanol- induced acute gastric mucosal lesions in mice

作　　者：叶惠惠[1] 刘河霞[1] 张咏梅[2] 吴克俭[3] 

机构地区：[1]徐州医学院研究生学院2009级消化内科专业,江苏徐州221002 [2]徐州医学院麻醉生理学教研室,江苏省麻醉学重点实验室,江苏徐州221002 [3]徐州医学院附属医院消化内科,江苏徐州221002

出　　处：《徐州医学院学报》2011年第1期36-39,共4页Acta Academiae Medicinae Xuzhou

基　　金：基金项目：江苏省教育厅自然科学基金（09KJD310009）;徐州医学院科研课题（09KJl7,09KJZ33）

摘　　要：目的研究引起小鼠急性乙醇性胃黏膜损伤的最佳乙醇浓度与容量，以建立稳定的小鼠急性乙醇性胃黏膜损伤模型。方法80只昆明小鼠随机分成正常对照组、生理盐水灌胃组、75％乙醇灌胃组、99．5％乙醇灌胃组，然后分别在1h、4h处死小鼠取胃，观察小鼠胃黏膜损伤情况。结果乙醇灌胃1h后，胃黏膜出现不同程度的损伤，其中99．5％乙醇0．2ml／10g对小鼠胃黏膜的损伤较重。99．5％乙醇0．2ml／10g灌胃4h与1h相比，胃黏膜损伤指数有所下降。随着乙醇浓度的增加与乙醇灌胃时间的延长小鼠的生存率并无显著变化。结论乙醇灌胃可致小鼠急性胃黏膜损伤，且以99．5％乙醇0．2m／／10g灌胃用来制备小鼠急性乙醇性胃黏膜损伤模型最佳。Objective To investigate the optimal ethanol concentration and capacity contributing to ethanol -in- duced acute gastric mucosal injury and to establish the stable model of ethanol - induced acute gastric mucosal injury in mice. Methods Eighty Kunming mice were randomly divided into control group, normal saline garage group, 75% eth- anol garage group, and 99.5% ethanol gavage group, followed by sacrifice of animals at 1 h and 4 h for removal of their stomachs and observation of the gastric mucosal lesions. Results The gastric mucosal lesions were evident following ga- rage of ethanol at the time point of 1 h in mice and 99.5% ethanol at 0.2 ml/lO g gavage induced much sever damage. The gastric mucosal lesions at 1 h were attenuated as compared to 4 h following 99.5% ethanol at 0.2 mE/10 g gavage. There wasn＇t significant change of survival rates with the concentration and duration of ethanol gavage in mice. Conclusion Ethanol garage can induce acute gastric mucosal injury in mice, with 99.5% ethanol at 0.2 ml/10 g as the optimal choice to establish the model of acute gastric mucosal injury.

关 键 词：乙醇 胃黏膜损伤 小鼠 

分 类 号：R573.9[医药卫生—消化系统] R338.2[医药卫生—内科学]