激光诱发荧光用于结肠肿瘤早期诊断  

Research on laser induced fluorescence using in the diagnosis of colon cancer

作　　者：谢洪波[1] 邓红梅[1] 郁道银[2] 

机构地区：[1]天津大学精密仪器与光电子工程学院,天津300072 [2]国家教育部光电信息科学开放实验室

出　　处：《激光杂志》1999年第5期21-23,共3页Laser Journal

摘　　要：激光诱导荧光法（ＬａｓｅｒＩｎｄｕｃｅｄ Ｆｌｕｏｒｅｓｃｅｎｃｅ ＬＩＦ）利用生物组织的自体荧光特性判断组织性质，能实时、无损地提供组织信息，从而区分正常与病变组织。本文采用固体可调谐激光器Ｃｒ：ＬｉＣＡＦ作为激发光源，在３６０ｎｍ ～４１０ｎｍ 波段范围选择多种波长对结肠癌和结肠正常组织标本进行ＬＩＦ测试，找出了能诱导结肠正常组织与癌变组织产生最大荧光光谱差异的最佳激发波长，并对３１ 组正常组织与病变组织标本进行测式，结果表明结肠正常组织与癌变组织自体荧光光谱存在明显差异。Laser induced fluorescence method,providing diagnostic information in a real time and nondestructive fashion,has the potential to detect colon dysplasia.In this paper,the auto fluorescence spectra were collected from normal colon and cancer tissues using 7 different excitation wavelength between 360nm and 410nm,which obtained from solid tunable laser (Cr:LiCAF),and significant difference of malignant and normal tissues was observed.The optimal excitation wavelength for differentiating normal and dysplastic colon laid at 380nm.31 tissue samples were studied under the 380nm excitation,and a discriminating equation was presented.

关 键 词：结肠癌 自体荧光光谱 激光诱发荧光 

分 类 号：R735.35[医药卫生—肿瘤] R730.4[医药卫生—临床医学]