孕期苯乙基异硫氰酸盐染毒对大鼠子代神经行为发育的影响  

Changes on neurobehavioral development of the offspring in rats prenataly exposed by phenethyl isothiocyanate

作　　者：支媛[1] 刘海波[1] 耿桂英[1] 于洲[1] 徐海滨[1] 

机构地区：[1]中国疾病预防控制中心营养与食品安全所,北京100021

出　　处：《卫生研究》2011年第1期36-39,42,共5页Journal of Hygiene Research

基　　金："十一五"国家科技支撑计划重大项目(No.2006BAK02A07);农业部科技行业科技项目(No.200903054-06)

摘　　要：目的研究大鼠孕期暴露于苯乙基异硫氰酸盐(PEITC)对子一代(F1)神经发育、早期行为及学习记忆能力等的影响。方法受孕大鼠于孕7～16天分别经口给予0、15、60和120mg/kg PEITC后,观察F1代断崖回避、平面翻正、前肢悬挂、听觉惊愕和嗅觉定位等早期行为发育指标的变化,并应用Y迷宫评价仔鼠学习记忆能力,对F1代大鼠大脑海马区神经元发育情况进行病理学检查。结果孕大鼠60和120mg/kg染毒剂量组,其F1代仔鼠平面翻正、断崖回避以及嗅觉定向等早期行为指标发育延迟,且运动学习能力下降;120mg/kg染毒剂量组F1代仔鼠海马区平均神经元密度为142±6.7,与阴性对照组相比有显著差异(P<0.05),且神经元损伤评分增至0～1级。结论大鼠孕期给予PEITC,可对F1代大鼠早期行为和学习记忆产生抑制和干扰;PEITC在15mg/kg剂量条件下无神经发育毒性和行为致畸作用。Objective To study the effect of rats prenatal exposed to phenethyl isothiocyanate（PEITC） on the postnatal growth and neurobehavioral development of rats.Methods Pregnant adult rats were randomly assigned to 4 groups,of which 3 groups were exposed to PEITC at doses of 15,60 and 120 mg /kg respectively from the 7th to 16th day of gestation,and their F1 pups were observed the impact of PEITC on the age acquisition of two reflexes（cliff avoidance,surface righting） and sensuous function（auditory startle and olfactory discrimination）,movement and coordination functions（forelimb hanging） and learning and memory（Y-maze test）.F1 pups’brain tissues were taken and neuronal morphology changes were observed with pathology staining.Results Compared to the control group,60 and 120mg /kg groups exposed inuterus caused the F1 rat pups’ cliff avoidance,surface righting and olfactory discrimination development delaying,and their learning and memory abilities were impaired.The increase to 0-1 level in histological grades and decrease to 142 ± 6.7 in neuronal density in the F1 pups of 120mg /kg group were proved.Conclusion PEITC exposure inuterus may impaire the F1 pups’neuro-behavior development and the abilities of learning,and the 15mg /kg level of PEITC has no effects on developmental neurotoxicity and behavioral teratogenesis.

关 键 词：神经行为 发育毒性 苯乙基异硫氰酸盐 孕期 染毒 

分 类 号：Q132.7[生物学—普通生物学] Q593.2