硫化氢对大鼠心脏骤停后海马组织bax和bcl-2表达的影响  

Effects of hydrogen sulfide on the expressions of bax and bcl - 2 in hippocampi of rats after cardiopulmonary resuscitation

作　　者：张自立[1] 蔺际龚[1] 廖晓星[2] 魏红艳[2] 李慧[2] 李欣[2] 刘荣[2] 胡春林[2] 戴刚[3] 

机构地区：[1]厦门大学附属第一医院急诊部,福建361003 [2]中山大学附属第一医院急诊科,广州510080 [3]中山大学卫生部辅助循环重点实验室,广州510080

出　　处：《中国急救医学》2011年第1期36-39,I0003,共5页Chinese Journal of Critical Care Medicine

基　　金：广州市科技攻关计划项目（No.200423-E0381）

摘　　要：目的 通过检测大鼠海马组织bcl-2和bax表达来探讨硫化氢（H2S）在心脏骤停（cardiac arrest, CA）后脑细胞凋亡中的作用.方法 雄性SD大鼠160只,初始选择108只随机均分为三组,建立CA/CPR模型.①第1组（36只）：在自主循环恢复（ROSC）后予以NaHS干预 ②第2组（36只）,在自主循环恢复（ROSC）后予以羟胺干预 ③第3组为常规复苏组（36只）.上述三组根据观察终点又均分为1、3和7 d三个亚组,每个亚组再等分两组分别做相关凋亡的免疫组化和RT-PCR检测.结果 ①三组经历CPR大鼠死亡及备用鼠使用的差别无统计学意义（P〉0.05） ②在CPR后,三组之间大鼠血清H2S浓度变化的差异有统计学意义（F=189.917,P=0.000）,在CPR后各时点上三组之间血清H2S浓度的差异均有统计学意义（P=0.000） ③在CPR后,三组之间大鼠海马组织CA1区bax和bcl-2蛋白表达累积光密度变化的差异有统计学意义（P〈0.05）,在CPR后各时点上三组之间海马组织CA1区bax和bcl-2蛋白表达累积光密度的差异均有统计学意义（P〈0.01） ④在CPR后,三组之间大鼠海马组织bax和bcl-2 mRNA相对表达量变化的差异有统计学意义（P=0.000）,在CPR后各时点上三组之间海马组织bax和bcl-2 mRNA相对表达量的差异有统计学意义（P〈0.01）.结论 在CPR后,H2S可能通过促进bcl-2和抑制bax表达来影响bcl-2/bax的平衡而参与神经细胞凋亡.Objective To examine the expression of bax and bcl -2 in the hippocampi of rats and investigate the effects of hydrogen sulfide （H2S） on neurons apoptosis after cardiopulmonary resuscitation （CPR）, Methods There were 160 male SD rats, the 108 rats were initially selected and randomly divided into 3 groups equally. Model of cardiac arrest were induced by transeutaneous electrical epieardium stimulation and standard CPR were offered in all the subjects. After ROSC, the First group were given sodium bisulflde,the second group were given hydroxylamine, and the third group were only given routine CPR. Above - mentioned the three groups were further divided three sub - groups based on the observed end point（ 1, 3 or 7 days） , then each sub -group were divided into two groups equally and given them relevant examination such as Immunohistochemistry or RT - PCR of bax and bel - 2. Results （1）Numbers of substitutes and mortality rate after ROSC were no significant difference among the three groups （ P 〉0.05 ）, （2）The variation trend of serum H2 S were different among the three groups （ P 〈0. 05 ）. There were significant differences in the serum levels of H2S among the three groups on 1, 3 and 7 days after ROSC （ P =0.000）. （3）The variation trend of the sum of integrated optical density （IOD） of bax and bcl - 2 in the hippocampal CA1 region were different among the three groups （ P 〈 0. 05 ）. On 1, 3 and 7 days after ROSC, the sum of IOD of bax and bcl - 2 in the hippocampal CA1 region among the three groups had statistic significance （ P 〈 0. 05 ）. And in every group, there were significant differences in the sum of IOD of bax and bcl - 2 in the hippocampal CA1 region among the three observed end points （P 〈0. 01 ）. （4）The variation trend of relative expression of bax and bcl- 2 mRNA in the hippocampi were different among the three groups （P 〈0. 05）. On 1,3 and 7days after CPR, the relative expression of bax mRNA and bcl -2 mRNA in the hippocampi amo

关 键 词：硫化氢 心肺复苏 凋亡 BAX bcl-2 

分 类 号：R542.2[医药卫生—心血管疾病]