灌注联合胰胆管结扎诱导大鼠急性胰腺炎  

Infusion combined with duct ligation inducing acute pancreatitis in rats

作　　者：王国俊[1] 李园[1] 王存[1] 丁思勤[1] 周总光[1] 

机构地区：[1]四川大学华西医院消化外科研究室及胃肠外科中心,成都610041

出　　处：《中华实验外科杂志》2011年第2期187-188,共2页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30830100）

摘　　要：目的 模拟急性胆源性胰腺炎的发病条件,制作一种符合临床特点的大鼠急性坏死性胰腺炎模型.方法 设计大鼠胰胆管单扎和双扎两种结扎方法,测压并用2%甘氨脱氧胆酸（GDCA）以35 cm H2O（1 cm H2O=0.098 kPa）压力恒压灌注5 min,8、16、24 h剖杀,观察急性胰腺炎的发生情况.结果 单扎组胆胰管压力为：（20.60±1.51）cm H2O,双扎组为：（29.37±0.87）cmH20.两组均诱导急性胰腺炎,于24 h表现出坏死性胰腺炎特征.病理学评分显示,随时间进程,胰腺炎逐渐加重（P＜0.01）.在各时间点,胆胰管双扎组炎症重于单扎组（P＜0.01）.结论 恒压灌注2%GDCA联合胰胆管单扎和双扎均可诱导出大鼠急性坏死性胰腺炎;胰管压力及其持续时间与急性胰腺炎发生、发展密切相关.Objective To establish a new acute necrotizing pancreatitis model in rats. Methods We designed single pancreatic bile duct ligation and double pancreatic bile duct ligation in rats, combined with retrogradely infusing 2% glycodeoxycholic acid （ GDCA）, to test duct pressure and observe the severity of acute pancreatitis at 8, 16 and 24 h, respectively. Results In two groups, the duct pressures were （20. 60 ± 1.51 ） cm H2O and （29. 37 ±0. 87） cm H2O, respectively. Both of the two methods could induce typical acute necrotizing pancreatitis at 24 h, the severity of which progressively developed with time course（P 〈0. 01 ）, at each time point, the severity of double duct ligation group was higher than single duct ligation group （ P 〈 0. 01 ）. Conclusion Retrogradely infusing 2% GDCA with constant pressure,combined with the two methods of pancreatic bile duct ligation, coude induce clinically relevant acute necrotizing pancreatitis in rats; the pancreatic duct pressure and its duration might be closely related to the arising and developing of acute pancreatitis.

关 键 词：急性胰腺炎 结扎 灌注 

分 类 号：R576[医药卫生—消化系统]