乌司他丁对细菌性腹膜炎大鼠小肠运动功能的保护作用  

Protective effect of Ulinastatin on intestinal motility in rats with bacterial peritonitis

作　　者：刘健[1] 赵战朝[1] 薛承锐[1] 

机构地区：[1]天津医科大学总医院外科,300052

出　　处：《中华实验外科杂志》2011年第2期233-235,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 探讨乌司他丁对细菌性腹膜炎大鼠小肠运动功能的保护作用及其机制.方法 将Wistar大鼠60只随机分成对照组、模型组和乌司他丁组.模型组和乌司他丁组制作细菌性腹膜炎动物模型,乌司他丁组于制模后30min静脉给予乌司他丁5万U/kg,其余组静脉给予等量生理盐水.观测各组大鼠小肠传输功能、小肠环行平滑肌条收缩能力、环行平滑肌细胞收缩反应及细胞内钙离子浓度（[Ca2＋]i）的变化;测定小肠平滑肌细胞核转录因子-κB（NF-κB）的活性;测定小肠肌层诱导型一氧化氮合酶（iNOS）mRNA的表达量和平滑肌细胞培养基中一氧化氮（NO）含量的变化.结果 乌司他丁组较模型组大鼠小肠传输明显改善.乙酰胆碱作用下,模型组和乌司他丁组小肠环行肌条收缩力分别为（0.75±0.06）、（1.87±0.29）g/mm2·s-1;平滑肌细胞收缩反应为（11.42±4.23）%、（37.22±10.08）%;[Ca2＋]i变化为（119±21）%、（205±49）%.模型组和乌司他丁组小肠肌层iNOS mRNA表达水平分别为1.38±0.41、0.58±0.17;平滑肌细胞培养基NO浓度为（98.34±19.62）、（32.72±7.98）μmol/L;平滑肌细胞NF-κB活性为303.25±47.18、170.22±29.62.结论 乌司他丁通过抑制小肠肌层NF-κB蛋白的活化,下调细菌性腹膜炎大鼠小肠肌层iNOS mRNA的表达,保护小肠的运动功能.Objective To explore the Protective effect and the mechanism of Ulinastatin （UTI） on intestinal motility in rats with bacterial peritonitis. Methods Sixty Wistar rats were divided randomly into control group, model group and UTI group （n=20 each）, and the latter two groups were used to replicate bacterial peritonitis model. Rats in UTI group received UTI injection （50 000 U/kg） via tail vein, while rats in other groups were given equal volume saline. Gut transits in vivo and intestinal circular muscle contractions were measured in an organ bath. The contractile response of dispersed circular smooth muscle cells and the changes of[Ca2＋]1 stimulated by aceylcholine were observed. Nuclear factor-κB （NF-κB） activity was examined by using electrophoretic mobility shift assay （EMSA）. Reverse transcription-polymerase chain reaction （RT-PCR） was used to detect the expression of inducible nitric oxide synthase （iNOS） mRNA within intestinal muscularis, and the level of nitric oxide （NO） in cell cultures was quantified. Results The gut transits in UTI group were improved obviously as compared with model group. In model group and UTI group, circular smooth muscle contractility stimulated by aceylcholine was （0. 75 ±0. 06 ） g/mm2· s - 1 and （ 1.87 ± O. 29 ） g/mm2· s - 1; contractile response of dispersed circular smooth muscle cells was （ 11.42 ± 4. 23 ） % and （ 37.22 ± 10. 08 ） %; changes of[Ca2＋]1 was （ 119 ± 21 ） % and （205 ±49）%. In model group and UTI group, the activity of NF-κB was 303.25 ±47.18 and 170. 22 ±29.62; levels of iNOS mRNA expression were 1.38 ±0. 41 and 0. 58 ±0. 17; NO production was （98. 34± 19. 62） μmol/L and （32. 72 ±7.98） μmol/L respectively. Conclusion UTI has the ability to protect the intestinal motility in rats with bacterial peritonitis through inhibiting the activity of NF-κB and down-regulating the expression of iNOS mRNA within intestinal muscularis.

关 键 词：乌司他丁 细菌性腹膜炎 小肠 NF-ΚB 一氧化氮 

分 类 号：R57[医药卫生—消化系统]