血管内皮细胞间连接蛋白VE-cadherin和β-连环蛋白在颅内动脉瘤中的表达  

The expression of VE-cadherin and β-catenin in the intracranial aneurysms

作　　者：李美华[1] 凌峻[1] 熊秋迎[2] 聂艳良[1] 

机构地区：[1]南昌大学第一附属医院神经外科,330006 [2]南昌大学第一附属医院病理科,330006

出　　处：《中华实验外科杂志》2011年第2期255-257,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30960397）;江西省自然科学基金资助项目（2009GZYO200）;江西省卫生厅课题基金资助项目（20091063）

摘　　要：目的 观察血管内皮钙黏蛋白（VE-cadherin）和β-连环蛋白（β-catenin）在SD大鼠和人颅内动脉瘤壁中的表达,探讨血管内皮细胞连接受损与颅内动脉瘤形成的关系.方法 建立肾性高血压诱导的SD大鼠颅内动脉瘤模型,获取8个SD大鼠颅内动脉瘤.另取15例颅内动脉瘤组织标本,应用免疫组织化学方法检测VE-cadherin在大鼠脑动脉瘤壁中的表达,以及VE-cadherin和β-catenin在人脑动脉瘤壁的表达.按照Martina的方法对血管内皮层免疫反应强度进行评分.结果 正常SD大鼠和人的脑动脉血管壁上内皮层完整,VE-cadherin呈棕褐色线样均匀表达.SD大鼠颅内动脉瘤壁上VE-cadherin表达减少,呈间断性非均匀分布.正常SD大鼠脑动脉血管壁和颅内动脉瘤壁免疫反应强度评分分别为2.40 ±0.55和1.38±0.51,两组间差异有统计学意义（t=3.41,P＜0.01）.人颅内动脉瘤壁内皮排列紊乱,VE-cadherin和β-catenin表达也明显减少,分布不均匀或表达缺失.正常脑动脉与颅内动脉瘤VE-cadherin和β-catenin免疫反应强度评分分别为2.60±0.54/1.33±0.49和2.80±0.45/1.33±0.49,VE-cadherin和β-catenin在两者间差异均有统计学意义（t分别为4.88和5.91;P＜0.01）.结论 颅内动脉瘤的形成过程中存在内皮细胞连接受损.Objective To verify whether vascular endothelial intercellular gap injury takes part in the formation of intracranial aneurysms by assaying the expression of vascular endothelial intercellular gap proteins VE-cadherin and β-catenin in the rat experimental intracranial aneurysms and human intracranial aneurysms. Methods Twenty Sprague-Dawley rats were used for establishing a model of hypertension-induced experimental intracranial aneurysm. Eight aneurysmal changes were found in the junction of the anterior cerebralartery （ACA） and the olfactort artery （OA） of rats, and immunohistochemistry was performed to detect the expression level of VE-cadherin. The expression of VE-cadherin and β-catenin was also assayed by using immunohistochemistry in fifteen human intracranial aneurysms. Results The VE-cadherin expression was reduced obviously in the intracranial aneurysms of rats as compared with the nomal cerebral artery （ 1.38 ±0. 51 vs 2.40 ±0. 55 ,P 〈0. 01 ）. In human intracranial aneurysms, the expression of VE-cadherin was lower than that in the human superficial temporal artery （ 1.33 ± 0. 49 vs 2. 60 ± 0. 54, P 〈0. 01 ）. And the expression of β-catenin was also reduced obviously as compared with the controls （ 1.33 ±0. 49 vs 2. 80 ± 0. 45 ,P 〈0. 01 ）. Conclusion Vascular endothelial intercellular gap injury may involve in the formation of intracranial aneurysms.

关 键 词：颅内动脉瘤 VE—cadherin Β-CATENIN 

分 类 号：R739.4[医药卫生—肿瘤]