甲氨蝶呤对人骨肉瘤MG-63细胞凋亡及28Livin和Caspase-3表达的影响  

Effect of MTX on apoptosis and expression of Livin and Caspase-3 of human osteosarcoma cell line MG-63

作　　者：王玉峰[1] 胡春吉[1] 柴利鹏[1] 王岩峰[1] 

机构地区：[1]中国医科大学附属第一医院骨科,沈阳110001

出　　处：《中华实验外科杂志》2011年第2期295-296,共2页Chinese Journal of Experimental Surgery

基　　金：辽宁省教育厅科技研究项目（2008557）

摘　　要：目的 观察甲氨蝶呤（MTx）对人骨肉瘤MG-63细胞凋亡及Livin和Caspase-3表达的影响.方法 体外培养骨肉瘤MG-63细胞株,用0、50、100、200和400μmol/L的MTX作用于骨肉瘤MG-63细胞24、48、72 h后,用噻唑蓝（MTT）比色法检测骨肉瘤MG-63细胞的增殖活性,用流式细胞仪测细胞的凋亡率,用Western blot检测不同浓度MTX作用于骨肉瘤MG-63细胞24 h后各组细胞的Livin和Caspase-3蛋白表达水平.结果 随MTX浓度增加骨肉瘤MG-63细胞的增殖活性降低,同时细胞的凋亡率增加（P＜0.05）,随MTX浓度增加各组细胞中Livin蛋白的表达降低,Caspase-3蛋白表达增加（P＜0.05）.结论 MTX诱导人骨肉瘤MG-63细胞凋亡,其机制可能与下调Livin表达继而上调Caspase-3表达有关.Objective To observe the effect of Methotrexate （MTX） on apoptosis and expression of Livin and Caspase-3 in human osteosarcoma cell line MG-63. Methods After treatment of MG-63 cells with MTX at different concentrations （0, 50, 100,200,400 μmol/L） for 24, 48 and 72 h, methyl thiazol tetrazolium（MTT） assay was used to observe the growth inhibition of MG-63. The apoptosis was assessed by flow cytometry. The protein expression of Livin and Caspase-3 was detected by Western blotting. Results When MTX was added, growth inhibition and increased apoptosis of MG-63 cells were detected,which was showed in a dose- and time-dependent manner. MTX also down-regulated the level of the protein expression of Livin （P〈0.05）, and elevated the protein expression of Caspase-3 （P〈0.05）. Conclusion MTX can induce apoptosis of MG-63 cells, by down-regulating Livin expression and subsequently up-regulating Caspase-3 expression.

关 键 词：甲氨蝶呤 骨肉瘤 CASPASE-3 

分 类 号：R738.1[医药卫生—肿瘤]