Isodihydroauroglaucin诱导的细胞凋亡可能与其诱导DNA损伤有关  

Apoptotic activity of isodihydroauroglaucin may correlate with its induction of DNA damage

作　　者：赵静[1,2] 姜■■■ 张新[1,2] 金东奎[2] 孙秉华 游松[1] 

机构地区：[1]沈阳药科大学生命科学与生物制药学院,辽宁沈阳110016 [2]韩国仁济大学生物药学化学学院 [3]釜庆国立大学化学院

出　　处：《沈阳药科大学学报》2011年第2期162-166,共5页Journal of Shenyang Pharmaceutical University

基　　金：韩国Brain Korea 21计划资助项目

摘　　要：目的对化合物2-(3E,5E-庚二烯基)-3,6-二羟基-5-(3-甲基-2-丁烯基)苯甲醛(isodihydroau-roglaucin)的抗癌活性进行研究。方法利用细胞毒实验测定isodihydroauroglaucin对人宫颈癌细胞(HeLa)的生长抑制作用;通过DNA含量分析和Annexin V-FITC/PI双染实验检测isodihydroauro-glaucin诱导的HeLa细胞死亡;利用单细胞凝胶电泳方法检测isodihydroauroglaucin对DNA的损伤作用。结果 Isodihydroauroglaucin对HeLa细胞的生长具有抑制作用,其IC50值为170μmol.L-1;DNA含量分析和Annexin V-FITC/PI双染实验表明isodihydroauroglaucin诱导HeLa细胞凋亡;isodi-hydroauroglaucin对DNA有损伤作用。结论 Isodihydroauroglaucin可能通过诱导DNA损伤诱导He-La细胞凋亡。Objective To investigate the anticancer activity of 2- （ 3E, 5E-heptadienyl ） -3,6-dihydroxy-5-（ 3- methyl-2-butenyl） benzaldehyde（isodihydroauroglaucin）. Methods Cytotoxicity assay was used to evaluate the anti-proliferation effect of isodihydroauroglaucin;DNA content analysis and Annexin V-FITC/PI dual staining assays were performed to determine the mode of HeLa cell death;single-cell gel electrophoresis was used to examine the DNA damage. Results Isodihydroauroglaucin inhibited HeLa cell growth, its IC50 value was about 170 μmol·L^-1 ;results from DNA content analysis and Annexin V-FITC/PI dual staining assays showed that isodihydroauroglaucin induced apoptosis of HeLa cells; isodihydroauroglaucin induced DNA damage in HeLa cells. Conclusions Isodihydroauroglaucin may induce apoptotic cell death by inducing DNA damage.

关 键 词：2-(3E 5E-庚二烯基)-3 6-二羟基-5-(3-甲基-2-丁烯基)苯甲醛(isodihydroauroglau—cin) 细胞凋亡 DNA损伤 抗癌活性 

分 类 号：Q253[生物学—细胞生物学] R96[医药卫生—药理学]