苦瓜多肽MC2的合成和降血糖活性(英文)  

Total Synthesis of MC2,a Peptide from Momordica Charantia and Its Hypoglycemic Activity

作　　者：金晶[1] 黄文龙[1] 饶光玲[2] 钱海[1] 张惠斌[1] 周金培[1] 王敬杰[1] 陈巍[1] 杜阔[1] 

机构地区：[1]中国药科大学新药研究中心,南京210009 [2]贵阳医学院药学院,贵阳550004

出　　处：《中国天然药物》2011年第1期58-60,共3页

基　　金：教育部科学技术研究重点项目(No.109086);"重大新药创制"科技重大专项(No.2009ZX09102-033)~~

摘　　要：目的:合成纯化从苦瓜中分离得到的多肽MC2,测试其药理活性。方法:应用Fmoc/t-Bu正交保护固相合成技术,在微波辅助条件下合成多肽。肽的粗品通过制备反相高效液相来纯化。用四氧嘧啶诱导糖尿病小鼠测试MC2的降糖活性。结果:在微波条件下能够快速高效的合成MC2,活性结果显示能够降低血糖。结论:合成了多肽MC2,具有降血糖的作用。AIM：To synthesize peptide MC2 isolated from Momordica Charantia and test its hypoglycemic activity.METHODS：The synthesis of MC2 was under microwave irradiation,using Fmoc/t-Bu orthogonal protection solid phase strategy.The crude peptide was purified by preparative RP-HPLC.The biological activity of MC2 was tested in the alloxan-induced diabetic mice.RESULTS：The peptide MC2 was rapidly and efficiently synthesized with a reduction of the blood glucose level.CONCLUSION：The MC2 was synthesized and was found to have hypoglycemic activity.

关 键 词：苦瓜 多肽 固相合成 微波 

分 类 号：R282.71[医药卫生—中药学]