黏着斑激酶和细胞外信号调节激酶在SACC-LM和SACC-83细胞中的表达及其意义  

Expressions of FAK and ERK in salivary gland adenoid cystic cancer cells SACC-LM and SACC-83

作　　者：张斌[1] 孙长伏[2] 于涛[1] 黄汉[1] 米磊[1] 

机构地区：[1]辽宁医学院附属第一医院口腔科,辽宁锦州121000 [2]中国医科大学附属口腔医学院口腔颌面外科,辽宁沈阳110001

出　　处：《吉林大学学报（医学版）》2011年第1期76-79,共4页Journal of Jilin University：Medicine Edition

基　　金：辽宁省教育厅高等学校科学研究项目资助课题(L2010315)

摘　　要：目的:检测黏着斑激酶(FAK)及细胞外信号调节激酶(ERK)在涎腺腺样囊性癌(SACC)细胞系SACC-LM和SACC-83中的表达,探讨其与SACC肺转移发生的关系。方法:选择停止在G1/S期的SACC-LM和SACC-83细胞,测定其细胞内FAK和ERK酶活力,采用Western blotting方法检测SACC-LM及SACC-83细胞中FAK及ERK蛋白表达,对其结果进行量化分析。结果:FAK及ERK在SACC-LM细胞中的活性均高于SACC-83细胞(P<0.01),SACC-LM细胞中FAK酶活性与ERK酶活性呈正相关关系(r=0.62,P<0.05)。Western blotting方法检测到在SACC-LM细胞中两种蛋白的表达水平均高于在SACC-83细胞中的表达水平(P<0.05)。结论:FAK及ERK可能参与了SACC的发生发展过程和转移信号转导通路,FAK及ERK蛋白表达变化与SACC的转移行为有关。Objective To detect the expressions of focal adhesion kinase（FAK） and extracellular signal-regulated protein kinase（ERK）in salivary adenoid cystic carcinoma（SACC） cells SACC-LM and SACC-83 and explore their relationship with occurrence of lung metastasis of SACC.Methods Western blotting was applied to detect the protein expressions of FAK and ERK in SACC-LM and SACC-83 in G1/S phase and the enzyme activities of FAK and ERK,the results were quantified.Results The activities of FAK and ERK in SACC-LM cells were higher than those in SACC-83 cells（P0.01）,the activity of FAK in SACC-LM cells had positive correlation with the activity of ERK（r=0.62,P0.05）.The Western blotting results showed that the protein expression levels of FAK and ERK in SACC-LM cells were higher than those in SACC-83 cells（P0.05）.Conclusion FAK and ERK may be involved in the occurrence and development process and metastasis signal conduction passage of SACC,the protein expressions of FAK and ERK concerns with the variation behavior of SACC.

关 键 词：黏着斑激酶 细胞外信号调节激酶 涎腺肿瘤 SACC-LM细胞 SACC-83细胞 蛋白表达 

分 类 号：R781.7[医药卫生—口腔医学]