视网膜节细胞投射到视交叉上核AVP神经元的研究——假狂犬病毒跨神经元追踪结合胶体金免疫电镜双重标记法  

A STUDY OF RETINAL GANGLION CELL PROJECTION TO AVP NEURON OF SUPRACHIASMATIC NUCLEUSDOUBLE LABELED TECHNIQUES OF PSEUDORABIES VIRUS ANTEROGRADE TRANSNEURONAL TRACING AND COLLOIDAL GOLD IMMUNOELECTRON MICROSCOPY

作　　者：张军[1] 欧可群[2] 吴良芳[2] 陈文玉[2] 操高原[2] 

机构地区：[1]中国科学院上海生理研究所,上海200031 [2]华西医科大学组织学研究室,成都610041

出　　处：《神经解剖学杂志》1999年第3期243-246,共4页Chinese Journal of Neuroanatomy

摘　　要：用９ 只成年ＳＤ 大鼠向单侧眼球玻璃体内注入假狂犬病毒３～４ μｌ，存活７２ ｈ 或９６ ｈ 后灌注、取材、Ｅｐｏｎ ８１２ 包埋，行免疫电镜胶体金标记。结果：（１）两个时间组视交叉上核内精氨酸血管加压素神经元被胶体金标记；（２）假狂犬病毒感染的视交叉上核神经元以含病毒颗粒为特征；（３）视交叉上核内少量神经元既含病毒颗粒又被精氨酸血管加压素胶体金标记。本研究结果首次证实视网膜节细胞与视交叉上核内的少数精氨酸血管加压素神经元之间有直接的突触联系，提示此类精氨酸血管加压素神经元可能直接接受来自视网膜的光信息。Our previous studies on level of light microscopy have proved that there might be a connection between retinal ganglion cell (RGC) and AVP neuron of suprachiasmatic nucleus(SCN). In order to prove it further, the present study applied double labeled techniques of PRV anterograde transneuronal tracing and colloidal gold immunoelectron microscopy. Nine SD rats received 3～4 μl PRV(Titer: 5×10 8 PFU/ml) by intraocular injection and perfused after 72 h(6 rats) and 96 h(3 rats) respectively. Tissue including SCN was taken and embedded in Epon 812. Ultrathin sections were incubated in primary antibody (AVP, 1∶1000) and goat anti rabbit IgG colloidal gold(1∶20). Results showed: (1)numerous AVP neurons of the SCN were labeled by gold particles both in groups at 72 h and 96 h; (2)some neurons of the SCN were infected by PRV both in groups at 72 h and 96 h; (3)a few PRV infected neurons of the SCN also contained gold particles in the cytoplasm both at 72 h and 96 h. This indicates that there is a direct synaptic connection between RGC and AVP neuron of the SCN. (Figures 1～6 on plate 45)

关 键 词：假狂犬病毒 免疫电镜 AVP神经元 视交叉上核 

分 类 号：R339.141[医药卫生—人体生理学]