蛇毒去整合素Kistrin对正常及糖尿病兔LECs增殖的抑制作用  

Inhibitive effect of disintegrin Kistrin on proliferation of lens epithelial cells in normal and diabetic rabbits

作　　者：谭少健[1] 韦琦[1] 姬翔[1] 陈金卯[1] 何剑峰[1] 梁皓[1] 

机构地区：[1]广西医科大学第一附属医院眼科,广西壮族自治区南宁市530021

出　　处：《眼科新进展》2011年第1期1-4,共4页Recent Advances in Ophthalmology

基　　金：国家自然科学基金资助(编号:30760268);广西自然科学基金资助(编号:桂科自0833141)~~

摘　　要：目的观察高血糖对兔眼晶状体摘出术后后囊膜混浊(posterior capsular opacification,PCO)的影响,探讨蛇毒去整合素Kistrin对晶状体上皮细胞(lens epithelial cells,LECs)增殖的抑制作用。方法制作正常血糖及糖尿病兔PCO模型,随机分组:正常血糖组分为A、B两组(每组3只兔3眼),糖尿病组分为C、D两组(每组3只兔3眼),A、C组为对照组(术毕前房注入林格氏液),B、D组为用药组(术毕前房注入80mg·mL-1的Kistrin2mL)。注药后在裂隙灯显微镜下观察4组PCO发生情况及形态并计算增殖指数(PI),注药后14d时取材,检测LECs增生细胞核抗原(proliferating cell nuclear antigen,PCNA)的表达。结果糖尿病兔PCO发生程度重于正常血糖兔;A组PI为0.25±0.05,B组0.03±0.01,C组0.86±0.04,D组0.65±0.08,A组PI明显小于C组(P=0.00),用药后B、D组PI均分别明显小于A、C组(均为P=0.01),但B组PI明显小于D组(P=0.00)。结论与正常血糖兔相比,糖尿病兔的PCO发生率较高,其LECs的增殖能力较强,Kistrin可明显抑制正常血糖及糖尿病兔的LECs增殖。Objective To observe the effects of hyperglycemia on posterior capsular opacification（PCO）after phacectomy in rabbits,and investigate the inhibitive effects of disintegrin Kistrin on lens epithelial cells（LECs）.Methods The PCO models were established in normal and diabetic rabbits,then the normal rabbits with PCO were randomly divided into group A and B（3 eyes of 3 rabbits in each group）,while the diabetic rabbits with PCO were randomly divided into group C and group D（3 eyes of 3 rabbits in each group）.Group A and group C were treated as blank groups（the Ringers solution were injected into their anterior chambers after the phacectomy）,group B and group D were set as Kistrin groups（2 mL Kistrin with 80 mg·L-1 was injected into the anterior chambers after the phacectomy）.The severities of PCO in all groups were evaluated by slit-lamp microscope after injection and the proliferative index（PI）was calculated.At 14 days after injection,the eyeballs were removed and the expressions of proliferating cell nuclear antigen（PCNA）in the LECs were detected by immunohistochemistry.Results The severities of PCO in diabetic rabbits were significantly higher than those in normal rabbits.The average PI of group A,B,C and D were 0.25±0.05,0.03±0.01,0.86±0.04,0.65±0.08 at 14 days after injection,respectively.Compared with group A,the PI of group C was significantly increased（P=0.00）.The PI in Kistrin groups（group B and D）were significantly decreased compared with blank groups（group A and C,both P=0.01）.However,the PI in group B was significantly less than that in group D（P=0.00）.Conclusions The severities of PCO and proliferation of LECs in diabetic rabbits are higher than those in normal rabbits.Kistrin can effectively inhibit the proliferation of LECs in either normal blood glucose or diabetic rabbits.

关 键 词：后囊膜混浊 糖尿病 去整合素 增殖细胞核抗原 

分 类 号：R776[医药卫生—眼科]