脉络膜黑色素瘤血管生成拟态的三维重建  

Three-dimensional reconstruction of vasculogenic mimicry in human uveal melanoma tissue

作　　者：陈雪[1] 艾筑明 Lu LEACH Robert FOLBERG 

机构地区：[1]北京大学第三医院眼科,北京市100191 [2]伊利诺伊州立大学芝加哥分校生物医学信息学院拟真医学实验室,美国芝加哥60612 [3]伊利诺伊州立大学芝加哥分校病理系,美国芝加哥60612

出　　处：《眼科新进展》2011年第1期20-23,共4页Recent Advances in Ophthalmology

摘　　要：目的血管生成拟态存在于原发性脉络膜黑色素瘤及其转移瘤中,经体内和体外实验均已证实这些血管生成拟态结构在肿瘤内部与其血循环系统相联系。本研究旨在进一步探求血管生成拟态的三维立体结构,以明确其与肿瘤血管的空间关系。方法原发性脉络膜黑色素瘤标本连续4μm厚切片共25张,层粘连蛋白荧光标记后以共聚焦显微镜观察,每张切片生成系列图像,连续25张切片的系列图像在三维拟真环境中配准叠加,从而获得立体结构重建。结果以层粘连蛋白为主要成分PAS染色阳性的血管生成拟态,二维平面中呈现为环形构成的血管生成拟态,在三维空间显示为薄片管形包绕黑色素瘤细胞聚集成的柱状条索,并形成多数分支和扭转。宿主血管在局部与这些层粘连蛋白阳性标记的柱状管相连。结论血管生成拟态可为造影或诊断提供新的给药途径。Objective Vasculogenic mimicry is present in tissue samples of primary aggressive human uveal melanomas and their metastases.Because these extravascular patterns connect to blood vessels and transmit fluid in vitro and in vivo,the three-dimensional configuration of these patterns has been the subject of considerable speculation.We designed methods to describe the three-dimensional configuration of vasculogenic mimicry in archiving human uveal melanoma tissues.Methods Twenty-five serial 4 μm-thick sections from primary uveal melanoma tissues were labeled with fluorescent-tagged laminin,examined by confocal microscopy to generate a z-series within each 4 μm-thick section,and the z-series from each section was stacked using an immersive three-dimensional environment to allow for precise alignment and compensation for distortion artifact.Results Vasculogenic mimicry that appeared to form loops in two dimensions were shown to represent thin wrappings around branching and twisting cylindrical groupings of melanoma cells.Blood vessels joined to some of these laminin-positive cylindrical wrappings.Conclusion Vasculogenic mimicry system may provide a novel delivery system for contrast and diagnostic agents.

关 键 词：黑色素瘤 血管生成拟态 三维重建 

分 类 号：R773.4[医药卫生—眼科]