IFN-γ对多发性骨髓瘤细胞上B细胞活化因子受体表达的影响及相关机制研究  

IFN-γ up-regulated the expression of BAFF-R in multiple myeloma cells through the NF-κB pathway

作　　者：申娴娟[1] 王跃国[1,2] 吴信华[3] 袁宏香[1] 祝文彩[1] 丛辉[1] 王惠民[1,2] 鞠少卿[1,2,3] 

机构地区：[1]江苏省南通大学附属医院医学检验中心,226001 [2]江苏省南通大学公共卫生学院医学检验研究室 [3]江苏省南通大学附属医院外科综合实验室

出　　处：《中华微生物学和免疫学杂志》2011年第1期20-24,共5页Chinese Journal of Microbiology and Immunology

基　　金：江苏省医学重点建设学科资助（XK200723）;南通市社会发展科技计划（$2007035）;江苏省六大人才高峰项目（2008095）

摘　　要：目的 探讨IFN-γ对多发性骨髓瘤（multiple myeloma,MM）细胞上B细胞活化因子受体（BAFF-R）的表达变化影响及相关机制.方法 应用RT-PCR和ELISA法检测IFN-γ诱导多发性骨髓瘤细胞BAFF-R的表达水平,观察NF-κB特异性抑制剂BAY11-7082对其mRNA和蛋白表达水平的影响;应用Western blot检测NF-κB在IFN-γ诱导的多发性骨髓瘤细胞反应中的活化程度.结果 IFN-γ以时间和剂量依赖方式上调多发性骨髓瘤细胞BAFF-R表达.BAY11-7082以剂量依赖方式在基因转录和翻译水平显著抑制IFN-γ诱导的BAFF-R表达.结论 NF-κB信号通路参与了多发性骨髓瘤细胞BAFF-R的表达,如何参与有待进一步研究.Objective To investigate the action and mechanism of NF-κB pathway in up-regulating B cell-activating factor receptor （BAFF-R） expression in multiple myeloma cells induced by IFN-γ.Methods Activated NF-κB were detected with Western blot, while the expression of BAFF-R were measured with RT-PCR and ELISA, and investigated the effect of BAY11-7082 on transcription of BAFF-R mRNA and translation of protein in multiple myeloma cells stimulated by IFN-γ. Results IFN-γ can induce the degradation of IκB-α in time-dependent and dosage-dependent manner, and up-regulated BAFF-R expression in multiple myeloma cells. BAY11-7082, an NF-κB inhibitor, inhibited not only the transcription of BAFF-R mRNA but also the protein of regulated by IFN-γin dosage-dependent manner. Conclusion NFκB may play an important role in high expression of BAFF-R in multiple myeloma cells induced by IFN-γ.

关 键 词：多发性骨髓瘤 BAFF-R受体 IFN-Γ NF-ΚB信号通路 

分 类 号：R733.3[医药卫生—肿瘤]